[#1994-10] Antitumor Activity and Immunological Property of Polysaccharides from the Mycelium of Liquid-Cultured Grifola frondosa, Cun Zhuang et al.

Antitumor Activity and Immunological Property of Polysaccharides from the Mycelium of Liquid-Cultured Grifola frondosa

Zhuang, C(a), Mizuno, T(b), Ito, H(c), Shimura, K(c), Sumiya, T(d), Kawade, M(d)

a) The United Graduate School of Agricultural Sciences, Gifu University, b) Faculty of Agriculture, Shizuoka University, c) School of Medicine, Mie University, d) Iwade Mushroom Institute, Japan

A systematic method for the fractionation and purification of antitumor polysaccharide fractions from the mycelium of liquid-cultured Grifola frondosa ( maitake ) was established. Twenty-three polysaccharide fractions were obtained. FI0-a-α, FI0-a-β, FA-1, and FA-2-b-α, in water-soluble fractions showed better antitumor activity against Sarcoma 180 mice, FIII-1-a, FIII-1-b, FIII-2-a, FIII-2-b, and FIII-2-c in water-insoluble fractions markedly inhibited the growth of Sarcoma 180 mice. In addition, administration of each of FI0-a, FIII-1-a, FIII-1-b, FIII-1-c, FIII-2-a, FIII-2-b, FIII-2-c to mice could cause an evident increase in antigenic C3 release from macrophages. These results suggest that active polysaccharide fractions, which were considered to be heteroglycan or heteroglycan-protein complexes, can depress or reduce tumor growth by activating the immune system as a biological response modifier.