[#1997-01] Leukocyte activation by (1-3)-β-D glucans, Yoshiyuki Adachi et al.
Leukocyte activation by (1-3)-β-D glucans
Yoshiyuki AdachiCA, Mitsuhiro Okazaki, Naohito Ohno and Toshiro Yadomae
We studied the activities of several kinds of β-glucans, including sonifilan, grifolan, Sclerotinia sclerotiorum glucan, laminarin and zymosan, on macrophages. Pre-culture of macrophages with inactive β-glucans rendered the cells unresponsive to subsequent stimulation with grifolan, suggesting a specific pathway in the β -glucan structure. The importance of protein C and phosphorylation of mitogen-activated protein kinase was demonstrated in the activation with grifolan or zymosan. Immunoprecipitation of complement receptor (CR 3), co-precipitated other proteins carrying phosphotyrosine residues in stimulation with Maitake grifolan. These data suggest that protein kinase C and tyrosine kinases are essential for signal transduction, and that CR3 might participate in the activation through interaction with other intracellular proteins.
