[#2009-06] Synergistic potentiation of interferon activity with maitake mushroom D-fraction on bladder cancer cells, Brandon Louie et al.
Synergistic potentiation of interferon activity with maitake mushroom D-fraction on bladder cancer cells
Brandon Louie, Srinivas Rajamahanty, John Won, Muhammad Choudhury and Sensuke Konno
Department of Urology, New York Medical College, Valhalla, NY, USA
To examine whether the combination of interferon (IFN)-α and maitake mushroom D-fraction (PDF), a bioactive mushroom extract, might potentiate the anticancer activity of IFN-α in bladder cancer T24 cells in vitro.
IFN-α2B was able to induce a significant ( ≈ 50%) growth reduction at 20 000 IU/mL, which further declined to ≈ 66% at 50 000 IU/mL. PDF had no effects up to 200 μg/mL, but there was an ≈ 20% and ≈ 53% growth reduction at 400 and 700 μg/mL, respectively. When the varying concentrations of IFN-α2B and PDF were combined, 10 000 IU/mL of IFN-α2B combined with 200 μg/mL of PDF resulted in an ≈ 75% growth reduction. This was accompanied by a G cell cycle arrest, shown by cell cycle analysis. Concurrently, DNA-PK activity in IFN-α2B/PDF-treated cells was almost three-fold higher than controls.
