[#2008-06] Grifola frondosa (Maitake Mushroom) Water Extract Inhibits Vascular Endothelial Growth Factor-Induced Angiogenesis Through Inhibition of Reactive Oxygen Species and Extracellular Signal-Regulated Kinase Phosphorylation, Jong-Suk Lee et al.

Grifola frondosa (Maitake Mushroom) Water Extract Inhibits Vascular Endothelial Growth Factor-Induced Angiogenesis Through Inhibition of Reactive Oxygen Species and Extracellular Signal-Regulated Kinase Phosphorylation

Jong-Suk Lee,¹ Byung Chul Park,¹ Yu Jin Ko,¹ Mi Kyoung Choi,¹ Han Gon Choi,¹
Chul Soon Yong,¹ Jae-Sung Lee,² and Jung-Ae Kim¹

¹College of Pharmacy and ²School of Food Technology and Food Service Industry,
Yeungnam University, Gyeongsan, Republic of Korea

ABSTRACT Grifola frondosa, a large edible mushroom also known as maitake, has been used as a health food for cen­turies in China and Japan. In the present study, we examined anti-angiogenic activity of a water extract of the fruiting body of G. frondosa (GFW). An in vivo angiogenesis assay using chick chorioallantoic membrane revealed that GFW (1–100 μg/mL) dose-dependently inhibited vascular endothelial growth factor (VEGF)-induced angiogenesis. In addition, GFW in­hibited VEGF-induced proliferation, chemotactic migration, and capillary-like tube formation of human umbilical vein en­dothelial cells (HUVECs) in a concentration-dependent manner. Upon stimulation by VEGF, HUVECs rapidly increased re­active oxygen species production, which was significantly blocked by the treatment with GFW. Moreover, phosphorylation of extracellular signal-regulated kinase 1/2, a downstream signaling molecule following VEGF receptor activation, was also inhibited by GFW. The results indicate that GFW effectively inhibit angiogenesis by blocking VEGF signaling and suggest that G. frondosa fruiting body may be a valuable medicinal food for treatment of angiogenesis-associated human diseases.