[#2006-05] Lysophosphatidylethanolamine ...

Lysophosphatidylethanolamine in Grifola frondosa as a neurotrophic activator via activation of  MAP kinase Atsuyoshi Nishina, Hirokazu Kimura, Akihiro Sekiguchi, Ryo-hei Fukumoto, Satoshi Nakajima and Shoei Furukawa ABSTRACT We found that Maitake (Grifola frondosa) extracts induced activation of mitogen-activated protein kinase (MAPK) in cultured PC12 cells, a cell line of rat pheochromocytoma cells. The active substance was isolated by a few chromatographic steps including high-performance liquid chromatography, and was identified to be lysophosphatidylethanolamine...

[#2006-04] Macrophage J774.1 cell is act...

Macrophage J774.1 cell is activated by MZ-Fraction (Klasma-MZ) polysaccharide in Grifola frondosa Masuda Y, Kodama N, Nanba H Department of Microbial Chemistry, Kobe Pharmaceutical University We previously reported that the (β-1,3)-branched (β-1,6)-glucan, MD-Fraction extracted from the fruit body of the edible maitake mushroom (Grifola frondosa) exhibits an antitumor effect. In this study, we separated a new polysaccharide from maitake, which we named Maitake MZ-Fraction (Klasma-MZ). MZ-Fraction is a β-glucan with a low molecular weight of about 20 000, which has a...

[#2006-03] Maitake Miyake, Naomi et al. ...

Low molecular weight maitake MD-Fraction (Klasma-MD) hydrolyzed with endo-β-1,6-glucanase of Trichoderma harzianum induces antitumor activities Miyake N, Masuda Y, Murata Y, Inoue M, Matuda K, Okamoto K, Nanba H, Kodama N Department of Microbial Chemistry, Kobe Pharmaceutical University We previously reported that MD-Fraction (Klasma-MD), a specific-structured β-glucan extracted from fruit bodies of maitake mushrooms (Grifola frondosa), can activate cellular immune systems and express antitumor effects. The molecular weight of the MD-Fraction ranged from 1,200,000 to...

[#2006-02] Maitake Gu, Chang-Qing et al....

Inhibition of hepatitis B virus by D-fraction from Grifola frondosa: Synergistic effect of combination with interferon-α in HepG2 2.2.15 Gu CQ, Li JW, Chao FH Institute of Health and Environmental Medicine, PR China In this study, D-fraction extracted from Grifola frondosa ( GF-D from maitake ) and its combination with human interferon alpha-2b (IFN) were investigated for the inhibitory effect on hepatitis B virus (HBV) in HepG2 2.2.15 cells (2.2.15 cells). HBV DNA and viral antigens were analyzed by a quantitative real-time polymerase chain reaction and end-point...

[#2005-08] Enhancement of cytotoxicity o...

Enhancement of cytotoxicity of NK cells by D-Fraction, a polysaccharide from Grifola frondosa Kodama N, Asakawa A, Inui A, Masuda Y, Nanba H. Department of Microbial Chemistry, Kobe Pharmaceutical University In innate immunity, activated natural killer (NK) cells attack and damage pathogens such as bacteria and virus without restriction by the MHC antigen. NK cells activated by IL-12 have been reported to recognize and kill tumor cells in perforin-mediated apoptosis. We have reported that D-Fraction, a polysaccharide extracted from the maitake mushroom (Grifola frondosa),...

[#2005-05] Characterization, Occurrence,...

Characterization, Occurrence, and Molecular Cloning of a Lectin from Gnfola frondosa: Jacalin-Related Lectin of Fungal Origin Yoshiho NAGATA,l‘t Masako YAMASHITA,’ Hiroaki HONDA,’ JUT1k0 AKABANE,’ Koichi UEHARA,’ Akihiro SArro,1 Futoshi SumtsA,1 Kozo NISHIBORI,2 and Yasuo OODAIRA2 ‘Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University, 2Section of Research and Development, Yukiguni-Maitake Co., Ltd. A lectin named GFL was isolated from the fruiting body of the basidiomycete mushroom Grifola frondosa, which...