[#2001-10] Nitric oxide-mediated antitum...

Nitric oxide-mediated antitumor activity induced by the extract from Grifola frondosa (Maitake mushroom) in a macrophage cell line, RAW264.7 Sanzen I, Imanishi N, Takamatsu N, Konosu S, Mantani N, Terasawa K, Tazawa K, Odaira Y, Watanabe M, Takeyama M, Ochiai H. Dept. of Clinical Nursing, Faculty of Medicine, Toyama Medical and Pharmaceutical University We have investigated D-fraction (MDF) extracted from Grifola frondosa ( Maitake mushroom) on the inducible nitric oxide synthase (iNOS)-mediated nitric oxide (NO) production in RAW264.7 (RAW) cells, a murine...

[#2001-09] A Possible Hypoglycemic Effec...

A Possible Hypoglycemic Effect of Maitake Mushroom on Type 2 Diabetes Patients Konno S, Tortorelis G, Fullerton SA, Samadi AA, Hettiarachchi J, Tazaki H Department of Urology, New York Medical College Two patients diagnosed with Type 2 diabetes had significantly elevated fasting blood glucose (FBG) levels even with administration of glibenclamide, a commonly-prescribed diabetes drug. Both patients were given Maitake mushroom caplets (500 mg) in daily doses, along with their glibenclamide, and experienced a sharp drop in blood glucose levels to within the normal range....

[#2001-08] Maitake SX-Fraction: Possible...

Maitake SX-Fraction: Possible Hypoglycemic Effect on Diabetes Mellitus Sensuke Konno, Ph.D. Diabetes mellitus is a metabolic dis­order characterized by persistent hyperglycemia affecting more than 16 million people in the United States and some 250 million people worldwide.1 The prevalence of diabetes is increasing by 4–5 percent every year with an estimated 40–45 percent of people over 65 years old at risk.2 To enhance peripheral insulin sensitivi­ty, pharmaceuticals, such as troglitazone8 and metformin,9,10 have been used. Although improved glycemic control with...

[#2001-07] Th1/Th2-Balancing Immunomodul...

Th1/Th2-Balancing Immunomodulating Activity of Gel-Forming (1→3)- β-Glucans from Fungi Maitake Yoko SUZUKI, Yoshiyuki ADACHI, Naohito OHNO, and Toshiro YADOMAE Laboratory of Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan The immunomodulating effects of various gel-forming (1→3)-β-glucans, grifolan (GRN), SSG, sonifilan (SPG) and alkaline-treated SPG (SPG-OH), on balancing helper T cell activity were examined in a murine model. Plasma from mice that were injected with GRN or SPG-OH and trinitrophenyl...

[#2001-06] Purification and Characteriza...

Purification and Characterization of an Aminopeptidase from the Edible Basidiomycete Grifola frondosa ( Maitake ) Toshikazu NISHIWAKI and Kiyoshi HAYASHI National Food Research Institute, Ministry of Agriculture, Forestry, and Fisheries, Ibaraki  Japan   An aminopeptidase was purified 178-fold from an ex­tract of Maitake ( Grifola frondosa ) by ammonium sulfate precipi­tation and a series of column chromatographies on phenyl-Toyopearl, Sephadex G-25, and Mono-Q. The molecular mass of the enzyme was estimated to be 27 kDa and 30 kDa by gel filtration and...

[#2001-05] Purification and Characteriza...

Purification and Characterization of Mushroom Proteases. 4. Maitake, Grifola frondosa Emi KENJO, Yoko YAMAGUCHI and Kosaku NODA Laboratory of Biochemistry, Department of Nutrition and Health Science, Faculty of Human Environmental Science, Fukuoka Women’s University, Japan   Recently, proteolytic enzymes are becoming increasingly important especially in the food industries.” Today the major uses of proteases occur in production and processing of diverse products such as cheese,” 6) baked goods,7‘  beer,9‘ I” flavor...