[#2001-07] Th1/Th2-Balancing Immunomodul...

Th1/Th2-Balancing Immunomodulating Activity of Gel-Forming (1→3)- β-Glucans from Fungi Maitake Yoko SUZUKI, Yoshiyuki ADACHI, Naohito OHNO, and Toshiro YADOMAE Laboratory of Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan The immunomodulating effects of various gel-forming (1→3)-β-glucans, grifolan (GRN), SSG, sonifilan (SPG) and alkaline-treated SPG (SPG-OH), on balancing helper T cell activity were examined in a murine model. Plasma from mice that were injected with GRN or SPG-OH and trinitrophenyl...

[#2001-06] Purification and Characteriza...

Purification and Characterization of an Aminopeptidase from the Edible Basidiomycete Grifola frondosa ( Maitake ) Toshikazu NISHIWAKI and Kiyoshi HAYASHI National Food Research Institute, Ministry of Agriculture, Forestry, and Fisheries, Ibaraki  Japan   An aminopeptidase was purified 178-fold from an ex­tract of Maitake ( Grifola frondosa ) by ammonium sulfate precipi­tation and a series of column chromatographies on phenyl-Toyopearl, Sephadex G-25, and Mono-Q. The molecular mass of the enzyme was estimated to be 27 kDa and 30 kDa by gel filtration and...

[#2001-05] Purification and Characteriza...

Purification and Characterization of Mushroom Proteases. 4. Maitake, Grifola frondosa Emi KENJO, Yoko YAMAGUCHI and Kosaku NODA Laboratory of Biochemistry, Department of Nutrition and Health Science, Faculty of Human Environmental Science, Fukuoka Women’s University, Japan   Recently, proteolytic enzymes are becoming increasingly important especially in the food industries.” Today the major uses of proteases occur in production and processing of diverse products such as cheese,” 6) baked goods,7‘  beer,9‘ I” flavor...

[#2001-04] Relationship between Solubili...

Relationship between Solubility of Grifolan, a Fungal 1,3-β-D-Glucan, and Production of Tumor Necrosis Factor by Macrophages in Vitro. Ishibashi K, Miura NN, Adachi Y, Ohno N, Yadomae T. Laboratory for Immunopharmacology for Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science Grifolan, GRN, is a fungal antitumor β-glucan isolated from Grifola frondosa ( maitake ). Various studies suggested that the underlying mechanism of the antitumor activity of GRN is strongly related to immune modulation. In the previous publication (Adachi et al.,...

[#2001-03] Addition of Maitake D-fractio...

Addition of Maitake D-fraction reduces the effective dosage of vancomycin for the treatment of Listeria-infected mice Kodama N, Yamada M, Nanba H. Department of Microbial Chemistry, Kobe Pharmaceutical University Maitake D-fraction, β1,6-glucan having β,3-branches, has been reported to activate the immune system of the host. To elucidate whether the D-fraction can reduce the clinical effective dosage of antibiotics in the treatment of opportunistic bacterial infection, we examined the effects of D-fraction on the treatment of Listeria monocytogenes-infected mice in...

[#2001-02] Maitake Extract and Their The...

Maitake Extract and Their Therapeutic Potential — A Review Mark Mayell Abstract Maiitake (Grifola frondosa) is the Japanese name for an edible fungus with a large fruiting body characterized by overlapping caps. It is a premier culinary as well as medicinal mushroom, Maitake is increasingly being recognized as a potent source of polysaccharide compounds with dramatic health-promoting potential. The most recent development is the MD-fraction, a proprietary maitake extract its Japanese inventors consider to be a notable advance upon the preceding D-fraction. The...