[#2000-05] Induction of apoptosis in hum...

Induction of apoptosis in human prostatic cancer cells with β-glucan (Maitake mushroom polysaccharide) Fullerton SA, Samadi AA, Tortorelis DG, Choudhury MS, Mallouh C, Tazaki H, Konno S. Department of Urology, New York Medical College PURPOSE: To explore more effective treatment for hormone-refractory prostate cancer, we investigated the potential antitumor effect of β-glucan, a polysaccharide of the Maitake mushroom, on prostatic cancer cells in vitro. MATERIALS AND METHODS: Human prostate cancer PC-3 cells were treated with various concentrations of the highly purified...

[#2000-04] Effects of maitake (Grifola f...

Effects of maitake (Grifola frondosa) polysaccharide on collagen-induced arthritis in mice Shigesue K, Kodama N, Nanba H. Department of Microbial Chemistry, Kobe Pharmaceutical University We recently reported the anti-hepatitis effect of a polysaccharide, designated as the D-fraction, extracted from maitake. Its effect includes immuno-regulating activities. We investigated the effect of the glucan in collagen-induced arthritis (CIA). The D-fraction was administered to CIA mice for 30 consecutive days. Arthritis development was observed from the 4th day after the second...

[#2000-03] Activities of Polysaccharides...

Activities of Polysaccharides Obtained from Grifola frondosa on Insulin-dependent Diabetes Mellitus Induced by Streptozotocin in Mice Kurushima, H, Kodama, N, Nanba, H Department of Microbial Chemistry, Kobe Pharmaceutical University In an experiment with C57BL/6J model mice having insulin-dependent diabetes mellitus (IDDM) induced by streptozotocin, the glucans obtained from Maitake fruit body were found to suppress activation and proliferation of macrophages and consequently to inhibit the generation of nitric oxide (NO) and IL (interleukin)-1β, which are β-cell...

[#1999-04] Activity of Edible Mushrooms...

 Activity of Edible Mushrooms Against the Growth of Human T4 Leukemic Cancer Cells, HeLa Cervical Cancer Cells and Plasmodium falciparum Lovy, A(a), Knowles, B(b), Labbe, R, Nolan(c), L(b) a) Institute of Biological Chemistry, Washington State University, b) Department of Environmental Health Sciences, University of Massachusetts, c) Food Science Department, University of Massachusetts Several edible Hymenomycete mushroom species have been screened as possible inhibitors of human T4 leukemic cancer cells, HeLa cervical cancer cells, Plasmodium falciparum (a pyrimethamine...

[#1999-03] Effect of maitake (Grifola fr...

Effect of maitake (Grifola frondosa) water extract on inhibition of adipocyte conversion of C3H10T1/2B2C1 cells Nakai R, Masui H, Horio H, Ohtsuru M. Department of Food Science and Nutrition, School of Human Environmental Science, Mukogawa Women’s University We investigated the effect of maitake (Grifola frondosa) water extract on inhibiting the conversion of C3H10T1/2B2C1 cells into adipocytes. Maitake water extract was fractionated by molecular sieve. Heat-labile compounds strongly inhibiting adipocyte conversion proved to occur in fractions of molecular weight of...

[#1999-02] Preparation of Antibody again...

Preparation of Antibody against Antitumor R-Glucan in Maitake ( Grifola frondosa ) and Its Application Masashi MIZUNO, Akio YAMAKAWA, Kenichiro MINATO, Sachiko KAWAKAMI, Shigenobu TATSUOKA, Hirofumi TERAI and Hironobu TSUCHIDA Graduate School of Science and Technology, Kobe University, Kobe , Japan Antibodies against an antitumor β-glucan purified from Grifola frondosa (GGF) were raised in the rabbit by subcutaneous immunization. Our antibodies reacted significantly with GGF by an ELISA inhibition assay. The antibodies did not recognize other polysaccharides such as...