[#1997-05] Sterol Constituents from the ...

Sterol Constituents from the Fruit Bodies of Grifola frondosa Ishizuka, T, Yaoita, Y, Kikuchi, M Tohoku College of Pharmacy Four new sterols, 5α, 6α-epoxy-(22E, 24R)-ergosta-8 (14), 22-diene-3β, 7β-diol (1), (22E, 24R)-ergosta – 8,22-diene-3β,5α,6β,7α-tetrol (2), (22E,24R)-ergosta-7,9(11), 22-triene-3β,5α,6β-triol (3) and 3β,5α,6β-trihydroxy-(22E,24R)-ergost-22-en-7-one (4), have been isolated from the fruit bodies of Grifola frondosa ( maitake ) together with fourteen known ones (5-18), of which two (5 and 6) are reported for the first time from a...

[#1997-04] Nitric Oxide Synthesis in Mur...

Nitric Oxide Synthesis in Murine Peritoneal Macrophages by Fungal β-Glucans Tomoe HASHIMOTO, Naohito OHNO, Yoshiyuki ADACHI, and Toshiro YADOMAE* Maitake Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, Japan. Fungal β-glucans have abilities to induce NO (nitric oxide) synthesis by macrophages in vivo, and the intensity of NO synthesis significantly varied dependent on the structure of β-glucans. The molecular mechanism of NO synthesis by β-glucans, however, has not been clarified in detail. To determine...

[#1997-03] Effect of Maitake D-Fraction ...

Effect of Maitake D-Fraction on Cancer Prevention Nanba, H  Kubo, K Department of Microbial Chemistry, Kobe Pharmaceutical University In this paper, the author investigates whether D-fraction is effective against chemically-induced cancer and against metastasis or recrudescence of MM-46 breast cancer. The known carcinogen, methylcholanthrene, was injected into mice. Subsequently, D-fraction or saline was administered. In the D-fraction-treated mice, carcinogenesis occurred in 31% of cases relative to 93% of cases in the control. In a similar experiment, tumor metastasis...

[#1997-02] Maitake D-Fraction: Healing a...

Maitake D-Fraction: Healing and Preventive Potential for Cancer Nanba, H Kobe Pharmaceutical University, Japan Author has been studying medicinal mushrooms for the last 15 years and has reported that of all mushrooms studied, Maitake Mushroom (Grifola frondosa) has the strongest activity in tumor growth inhibition both in administered orally and intraperitoneally. In this report, Maitake extract D-fraction was investigated to determine its effectiveness not only on the inhibition against tumors already growing, but also on the inhibition of formation of the secondary focus...

[#1997-01] Leukocyte activation by (1-3)...

Leukocyte activation by (1-3)-β-D­ glucans Yoshiyuki AdachiCA, Mitsuhiro Okazaki, Naohito Ohno and Toshiro Yadomae   We studied the activities of several kinds of β-glucans, including sonifilan, grifolan, Sclerotinia sclerotiorum glucan, laminarin and zymosan, on macrophages. Pre-culture of macrophages with inactive β-glucans rendered the cells unresponsive to subsequent stimulation with grifolan, suggesting a specific pathway in the β -glucan structure. The importance of protein C and phosphory­lation of mitogen-activated protein kinase was demon­strated...

[#1996-05] Anti Diabetic Mechanism of Ma...

Anti Diabetic Mechanism of Maitake (Grifola frondosa) Kubo, K, Nanba, H Department of Microbial Chemistry, Kobe Pharmaceutical University Glucose Tolerance Tests were conducted on KK-A mice, model animals of NIDDM (non-insulin dependent diabetes mellitus). The elevated blood glucose levels after 15 minutes and 30 minutes of Maitake-fed group were 0.64 times and 0.76 times those of the control group respectively, indicating inhibition of a significant blood glucose increase. Insulin receptor capability of liver cells then was examined using the liver perfusion method....