[#1995-06] Results of Non-Controlled Cli...

Results of Non-Controlled Clinical Study for Various Cancer Patients Using Maitake D-Fraction Nanba, H Department of Microbial Chemistry, Kobe Pharmaceutical University A non-randomized clinical study using D-fraction and Maitake crude tablets was conducted to investigate if those are effective against cancer patients as they were against animals. The study was carried out with 165 patients with advanced cancer, stage III-IV, 25-65 years old. Most of the patients receiving the Maitake treatment claimed improvement of overall symptoms. The highest response rates were seen...

[#1995-05] Structure-activity relationsh...

Structure-activity relationship of (1->3)-β-D-glucans in the induction of cytokine production from macrophages, in vitro Okazaki M, Adachi Y, Ohno N, Yadomae T. Laboratory of Immunopharmacology of Microbial Products School of Pharmacy, Tokyo University of Pharmacy and Life Science In a previous study, we reported that one of the gel-forming (1->3)-β-D-glucans, grifolan (from Maitake  Grifola frondosa, GRN), stimulated cytokine production from macrophages in vitro. However, several other gel-forming (1->3)-β-D-glucans, such as sonifilan (SPG) and SSG, did not...

[#1995-04] Maitake: Grifolan-Ohno, Naohi...

Enhancement of LPS triggered TNF-α (tumor necrosis factor-α) production by (1->3)-β-D-glucans in mice Ohno N, Asada N, Adachi Y, Yadomae T. Tokyo College of Pharmacy Effects of (1->3)-β-D-glucans on tumor necrosis factor-α (TNF-α ) production in mice in vivo were investigated with or without triggering stimulation of lipopolysaccharide (LPS). Administration of grifolan (GRN) (100-250 μg/mouse) obtained from Grifola frondosa ( maitake ), did not elevate the TNF-α concentration in serum, but significantly elevated LPS (10 μg/mouse)-elicited TNF-α...

[#1995-03] Characterization of a Thermos...

Characterization of a Thermostable Lysine-Specific Metalloendereptidase from the Fruiting Bodies of a Basidiomycete, Grifola frondosa Takashi Nonaka, Hiroko Ishikawa, Yoichi Tsumuraya, Yohichi Hashimoto, Naoshi Dohmae, and Koji Takio Department of Biochemistry, Faculty of Science, Saitama University, Saitama, Japan; and Division of Biomolecular Characterization, The Institute of Physical and Chemical Research (RIKEN), Saitama, Japan   A zinc-metalloendopeptidase, MEP, capable of catalyzing specific cleavage of acyl-lysine bonds (-X-Lys-) in polypeptides has been...

[#1995-02] Inactivation of (1-3)-β-D-Glu...

Inactivation of (1-3)-β-D-Glucan in Mice Toshihide MIURA, Naohito OHNO, Masahiro SUDA, Noriko N. MIURA, Shigehiko SHIMADA, and Toshiro YADOMAE Tokyo University of Pharmacy and Life Science (formerly Tokyo College of Pharmacy), Tokyo Japan and Kitasato University Hospital, Department of Pharmacy, Kanagawa, Japan. Intraperitoneally or intravenously administered (1–>3)-β-D-glucan remained in the liver and spleen, for a long time without major structural changes, but the priming activity to lipopolysaccharide (LPS)-triggered tumor necrosis factor-α (TNF-α)...

[#1995-01] Activity of Maitake D-Fractio...

Activity of Maitake D-Fraction to Inhibit Carcinogenesis and Metastasis Nanba, H Department of Microbial Chemistry, Kobe Pharmaceutical University Of all the medicinal mushrooms studied by the author, the Maitake mushroom (Grifola frondosa) has the strongest antitumor activity in tumor growth inhibition both when administered orally and intraperitoneally. In fact, most of the other mushroom extracts are reported ineffective when given orally. In this report, the author investigated Maitake’s activities on the inhibition of carcinogenesis and metastasis in mouse...