[#1994-03] Preparation and Antigen Speci...

Preparation and Antigen Specificity of an Anti-(1->3)-β-D-glucan Antibody Yoshiyuki ADACHI, Naohito OHNO, and Toshiro YADOMAE Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, Tokyo, Japan. Antibody for (1-6)-branched (1-3)-β-D-glucan was prepared using rodents. An antitumor (1-6)-β-monoglucosyl branched (1-3)-β-D-glucan (GRN: grifolan from maitake ) was conjugated with bovine serum albumin and used as an immunogen. The antibody titer in serum was determined by ELISA using biotin-conjugated GRN. Administration of the antigen raised...

[#1993-04] Anti-Hypertensive Effect by t...

Anti-Hypertensive Effect by the King of Mushrooms Nanba, H Kobe Women’s College of Pharmacy The fungi which belong to Basidiomycetes have been used as herbal medicines for a long time. It has been found that shiitake (Lentinus edodes) and mannentake or reishi (Ganoderma lucidum) are effective against essential hypertension. The author was quite curious about the hypotensive action of Maitake (Grifola frondosa), another mushrooom belonging to basidiomycetes. In fact, the finding of this activity was a result of studying the antitumor activity of Maitake. In this...

[#1993-03] Purification of a copper bind...

Purification of a copper binding peptide from the mushroom Grifola frondosa and its effect on copper absorption Iwao Shimaoka,* Junko Kodama,* Kohsuke Nishino,t and Yoshinori Itokawa* *Department of Hygiene, Faculty of Medicine, Kyoto University; and tDepartment of Home Economics, Dohshisha Women’s College, Kyoto, Japan The present studies were undertaken to investigate the effect of a copper binding peptide on copper bioavailability. This peptide was extracted from the fruit bodies of the mushroom, Grifola frondosa ( maitake ), and purified to a single peptide...

[#1993-02] Application of limulus test (...

Application of limulus test (G pathway) for the detection of different conformers of (1->3)-β-D-glucans Nagi N, Ohno N, Adachi Y, Aketagawa J, Tamura H, Shibata Y, Tanaka S, Yadomae T. Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy The reactivity of factor G mediated coagulation pathway in limulus amebocyte lysate which is triggered by (1->3)-β-D-glucans is thought to depend on the structure of the glucans, especially on the ultrastructure: triple helix, single helix and random coil. We used Sonifilan (SPG) and grifolan (GRN...

[#1991-05] Chemical Structure of Water-S...

Chemical Structure of Water-Soluble Alpha-Glucan in the Fruit Body of Grifola Frondosa Wei, B, Wu, Y, Okumura, N, Yamaguchi, R, Ueno, Y, Kato, K Department of Food Science, Gifu University Alpha-glucan composed of only D-glucose was extracted with boiling water from the fruit body of Grifola frondosa ( maitake ) and purified through the columns of DEAE-cellulose (borate form) and Sepharose CL-4B. The polysaccharide was found to be a glycogen-type from the results of methylation study and enzymic hydrolysis. About 1.3 ~106 of weight-average molecular weight and 5~40...

[#1991-04] Preparation and Biological Ac...

Preparation and Biological Activities of Sulfated Derivatives of (1-3)-β-D-Glucans Tatsuya SUZUKI, Naohito OHNO, Yoshiyuki ADACHI,* Alicia Fernandez CIRELLI, Javiar A. COVIAN,** and Toshiro YADOMAE* Tokyo College of Pharmacy, * 1432-1 Horinouchi, Hachioji, Tokyo, 192-03, Japan and Univer­sidad de Buenos Aires, ** Pabellon II, Ciudad Universitaria, 1428 Buenos Aires, Argentina Sulfated derivatives of (1–>3)β-D-glucans with different degree of branching (DB) i.e., curdlan (linear, DB 0), grifolan (from maitake ) (6-0-substituted by β-D-glucose at every third residue...