[#1993-02] Application of limulus test (...

Application of limulus test (G pathway) for the detection of different conformers of (1->3)-β-D-glucans Nagi N, Ohno N, Adachi Y, Aketagawa J, Tamura H, Shibata Y, Tanaka S, Yadomae T. Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy The reactivity of factor G mediated coagulation pathway in limulus amebocyte lysate which is triggered by (1->3)-β-D-glucans is thought to depend on the structure of the glucans, especially on the ultrastructure: triple helix, single helix and random coil. We used Sonifilan (SPG) and grifolan (GRN...

[#1991-05] Chemical Structure of Water-S...

Chemical Structure of Water-Soluble Alpha-Glucan in the Fruit Body of Grifola Frondosa Wei, B, Wu, Y, Okumura, N, Yamaguchi, R, Ueno, Y, Kato, K Department of Food Science, Gifu University Alpha-glucan composed of only D-glucose was extracted with boiling water from the fruit body of Grifola frondosa ( maitake ) and purified through the columns of DEAE-cellulose (borate form) and Sepharose CL-4B. The polysaccharide was found to be a glycogen-type from the results of methylation study and enzymic hydrolysis. About 1.3 ~106 of weight-average molecular weight and 5~40...

[#1991-04] Preparation and Biological Ac...

Preparation and Biological Activities of Sulfated Derivatives of (1-3)-β-D-Glucans Tatsuya SUZUKI, Naohito OHNO, Yoshiyuki ADACHI,* Alicia Fernandez CIRELLI, Javiar A. COVIAN,** and Toshiro YADOMAE* Tokyo College of Pharmacy, * 1432-1 Horinouchi, Hachioji, Tokyo, 192-03, Japan and Univer­sidad de Buenos Aires, ** Pabellon II, Ciudad Universitaria, 1428 Buenos Aires, Argentina Sulfated derivatives of (1–>3)β-D-glucans with different degree of branching (DB) i.e., curdlan (linear, DB 0), grifolan (from maitake ) (6-0-substituted by β-D-glucose at every third residue...

[#1991-03] Enhancement of clot formation...

Enhancement of clot formation of human plasma by β-glucans Ohno N, Suzuki T, Saito K, Yadomae T. Tokyo College of Pharmacy Effects of (1->3)-β-D-glucans on clot formation of human plasma were examined. Gel forming 6 branched (1->3)-β-D-glucans, SSG, branched at every other main chain glucosyl unit, grifolan (GRN, from maitake ) and schizophyllan, branched at one third of main chain glucosyl unit, enhanced clot formation of human plasma. GRN derivatives also increased the contents of clot, but less effectively than GRN. This effect was dependent on dose, molecular...

[#1991-02] Oxidative Degradation of an A...

Isao NONO, Naohito OHNO,* Akiyoshi MASUDA, Shozo OIKAWA,** and TosOxidative Degradation of an Antitumor (1-3)-β-D-Glucan, Grifolanhiro YADOMAE* Tokyo College of Pharmacy, * 1432-1 Horinouchi, Hachioji, Tokyo, 192-03, Japan and Nippon Beet Sugar Mfg., Co., Ltd., ** 2-3-13 Kyobashi, Chuo-ku, Tokyo, 104, Japan A (1-3)-β-D-glucan, grifolan (GRN, from maitake ), recovered from the peritoneal cavity after 1 d from i.p. injection contained a significant amount of anionic metabolite(s) having lower Mr than the parent GRN. In parallel with this observation, GRN induced peritoneal...

[#1990-05] Isolation and characterizatio...

Isolation and characterization of a lectin from Grifola frondosa fruiting bodies Kawagishi H, Nomura A, Mizuno T, Kimura A, Chiba S. Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University An N-acetylgalactosamine-specific lectin (GFL) was isolated from Grifola frondosa ( maitake ) fruiting bodies by affinity chromatographies on acid-treated Sepharose CL-4B and then GalNAc-Toyopearl. The isolated lectin agglutinated all types of erythrocytes equally. Molecular masses estimated by gel filtration under various buffers and matrices varied from...