[#1990-04] An Alkali-Soluble Alpha-D-Glu...

An Alkali-Soluble Alpha-D-Glucan from the Fruiting Body of Grifola frondosa Kato, K, Kawaguchi, M, Kumada, K, Yamauchi, R, Ueno, Y Department of Agricultural Chemistry, Gifu University Previously, we reported studies of the polysaccharides extracted from the fruiting body of the basidiomycete Grifola frondosa ( maitake ) with 10% aqueous zinc choride (designated F4) and 10% aqueous sodium hyroxide (designated F7 and F8). In continuation of this work, we now describe the isolation of an alkali-soluble alpha-D-glucan present in the fruiting body. The structure of the glucan...

[#1990-03] Antitumor Effect of Orally A...

Antitumor Effect of Orally Administered Extracts from Fruit Body of Grifola frondosa ( Maitake ) Yamada, Y, Nanba, H, Kuroda, H Laboratory of Microbiology, Kobe Women’s College of Pharmacy We conducted a footpad swelling test to clarify whether a delayed-type hypersensitivity (DTH) reaction against tumor antigen is potentiated by oral administration of D-fraction extracted and purified from fruit body of Grifola frondosa (maitake) with a ratio of β-glucan to protein of 7:3. When the antigen was injected in D-fraction administered tumor-bearing mice (D-mice), footpad...

[#1990-02] Change of biological activiti...

Change of biological activities of (1-3)-β-D-glucan from Grifola frondosa upon molecular weight reduction by heat treatment Adachi Y, Ohno N, Ohsawa M, Oikawa S, Yadomae T. Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy Changes of biological activities manifested by (1-6)-branched (1-3)-β-D-glucans (from maitake ) of various molecular weights obtained by heat treatment of the corresponding intact β-glucan at 150 degrees C (HD-LE) were examined. The activities assessed in this study were as follows: an antitumor activity, activation of...

[#1990-01] Macrophage Activation in Vitr...

Macrophage Activation in Vitro by Chemically Cross-Linked (1-3)-β-D-Glucans Yoshiyuki ADACHI,a Naohito °two,’ Masumi OHSAWA, Shozo OEKAWA and Toshiro YADOMAE Tokyo College of Pharmacy, Horinouchi, Hachioji, Tokyo 192-03, Japan and Nippon Beet Sugar Mfg. Co., Ltd., Kyobashi, Chuo-ku, Tokyo 104, Japan. Received September 11, 1989 The chemical cross-linking of soluble (1 -3)-β-D-glucans (from maitake ) having molecular weights of 21000 (CL 3 h) and 6400 (CL 6 h), and laminarin (CL LAMI), which showed negligible biological activity, by epichlorohydrin provided...

[#1989-04] Antitumor and Immunomodulatin...

Antitumor and Immunomodulating Activities of a β-Glucan Obtained from Liquid-Cultured Grifola frondosa Iwao SUZUKI,  Koichi HASHIMOTO,  Shozo OIKAWA,  Kichiro SATO  Masumi OSAWA and Toshiro YADOMAE Tokyo College of Pharmacy, Horinouchi, Hachioji, Tokyo 192-03, Japan and Nippon Beet Sugar Mfg., Co., Ltd., Chuo-ku, Tokyo 104, Japan. The effects of the β-1,3-glucan, LELFD, obtained from liquid-cultured mycelium of Grifola frondosa ( maitake ), on the growth of syngeneic tumors and immune responses in mice were examined. In Meth A or IMC solid tumor systems, LELFD...

[#1989-03] Modification of Immunostimula...

Modification of Immunostimulating Activities of Grifolan by the Treatment with (1—.3)-p-D-Glucanase Isao NONO, Naohito OHNO,* Masumi OHSAWA, Shozo OIKAWA,** and Toshiro YADOMAE* Tokyo College of Pharmacy, * Horinouchi, Hachioji, Tokyo, 192-03, Japan and Nippon Beet Sugar Mfg., Co., Ltd., ** Chuo-ku, Tokyo, 104, Japan   We have developed a method to control the effective time period of (1->3)-β-D-glucan in vivo by using (1->3)-β-D-glucanase and found that grifolan LE (GRN), from maitake ) , a (1->3)-β-D-glucan, administered intraperitoneally to the host...