[#1991-03] Enhancement of clot formation...

Enhancement of clot formation of human plasma by β-glucans Ohno N, Suzuki T, Saito K, Yadomae T. Tokyo College of Pharmacy Effects of (1->3)-β-D-glucans on clot formation of human plasma were examined. Gel forming 6 branched (1->3)-β-D-glucans, SSG, branched at every other main chain glucosyl unit, grifolan (GRN, from maitake ) and schizophyllan, branched at one third of main chain glucosyl unit, enhanced clot formation of human plasma. GRN derivatives also increased the contents of clot, but less effectively than GRN. This effect was dependent on dose, molecular...

[#1991-02] Oxidative Degradation of an A...

Isao NONO, Naohito OHNO,* Akiyoshi MASUDA, Shozo OIKAWA,** and TosOxidative Degradation of an Antitumor (1-3)-β-D-Glucan, Grifolanhiro YADOMAE* Tokyo College of Pharmacy, * 1432-1 Horinouchi, Hachioji, Tokyo, 192-03, Japan and Nippon Beet Sugar Mfg., Co., Ltd., ** 2-3-13 Kyobashi, Chuo-ku, Tokyo, 104, Japan A (1-3)-β-D-glucan, grifolan (GRN, from maitake ), recovered from the peritoneal cavity after 1 d from i.p. injection contained a significant amount of anionic metabolite(s) having lower Mr than the parent GRN. In parallel with this observation, GRN induced peritoneal...

[#1990-05] Isolation and characterizatio...

Isolation and characterization of a lectin from Grifola frondosa fruiting bodies Kawagishi H, Nomura A, Mizuno T, Kimura A, Chiba S. Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University An N-acetylgalactosamine-specific lectin (GFL) was isolated from Grifola frondosa ( maitake ) fruiting bodies by affinity chromatographies on acid-treated Sepharose CL-4B and then GalNAc-Toyopearl. The isolated lectin agglutinated all types of erythrocytes equally. Molecular masses estimated by gel filtration under various buffers and matrices varied from...

[#1990-04] An Alkali-Soluble Alpha-D-Glu...

An Alkali-Soluble Alpha-D-Glucan from the Fruiting Body of Grifola frondosa Kato, K, Kawaguchi, M, Kumada, K, Yamauchi, R, Ueno, Y Department of Agricultural Chemistry, Gifu University Previously, we reported studies of the polysaccharides extracted from the fruiting body of the basidiomycete Grifola frondosa ( maitake ) with 10% aqueous zinc choride (designated F4) and 10% aqueous sodium hyroxide (designated F7 and F8). In continuation of this work, we now describe the isolation of an alkali-soluble alpha-D-glucan present in the fruiting body. The structure of the glucan...

[#1990-03] Antitumor Effect of Orally A...

Antitumor Effect of Orally Administered Extracts from Fruit Body of Grifola frondosa ( Maitake ) Yamada, Y, Nanba, H, Kuroda, H Laboratory of Microbiology, Kobe Women’s College of Pharmacy We conducted a footpad swelling test to clarify whether a delayed-type hypersensitivity (DTH) reaction against tumor antigen is potentiated by oral administration of D-fraction extracted and purified from fruit body of Grifola frondosa (maitake) with a ratio of β-glucan to protein of 7:3. When the antigen was injected in D-fraction administered tumor-bearing mice (D-mice), footpad...

[#1990-02] Change of biological activiti...

Change of biological activities of (1-3)-β-D-glucan from Grifola frondosa upon molecular weight reduction by heat treatment Adachi Y, Ohno N, Ohsawa M, Oikawa S, Yadomae T. Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy Changes of biological activities manifested by (1-6)-branched (1-3)-β-D-glucans (from maitake ) of various molecular weights obtained by heat treatment of the corresponding intact β-glucan at 150 degrees C (HD-LE) were examined. The activities assessed in this study were as follows: an antitumor activity, activation of...