[#1986-05] Antitumor activity of a β-1,3...

Antitumor activity of a β-1,3-glucan obtained from liquid cultured mycelium of Grifola frondosa Ohno N, Adachi Y, Suzuki I, Oikawa S, Sato K, Ohsawa M, Yadomae T. Tokyo College of Pharmacy The antitumor activity of a branched β-1,3-glucan “grifolan LE” purified from liquid cultures of Grifola frondosa ( maitake ) (Ohno et al. Chem. Pharm. Bull., 34, 1709-1715, 1986) was examined on an allogeneic murine tumor system. By intraperitoneal (i.p.) administration (100-200 μg/mouse/d X 5) at days 1 to 9 from the tumor transplantation, grifolan LE showed marked...

[#1986-04] Effect of Activation or Block...

Effect of Activation or Blockade of the Phagocytic System on the Antitumor Activity of Grifolan NAOHITO OHNO,a MAMI HAYASHI,a IWAO SUZUKI,a SHOZO OIKAWA,b KICHIRO SATO,b YOSHIYUKI Suzuiu,b and TOSHIRO YADOMAE*‘ a Tokyo College of Pharmacy,a and Nippon Beet Sugar Mfg., Co., Ltd.,b The effect of activation or blockade of the phagocytic system on the antitumor activity of grifolan from Maitake was investigated. The activity of grifolan was abrogated by treatment of mice with a blocker of the phagocytic system, i.e., carrageenan, colloidal carbon, or trypan blue. The...

[#1986-03] Fractionation of Acidic Antit...

Fractionation of Acidic Antitumor β-Glucan of Grifola frondosa by Anion-Exchange Chromatography Using Urea Solutions of Low and High Ionic Strengths NAOHITO OHNO,a KAZUYOSHI IINO,a SHOZO OEKAWA,b KICHIRO SATO,b MASUMI OHSAWA,b and TOSHIRO YADOMAE*‘ a Tokyo College of Pharmacy,’ Horinouchi, Hachioji, Tokyo 192-03, Japan and Nippon Beet Sugar Mfg. Co., Ltd.,’ Kyobashi, Chuo-ku, Tokyo 104, Japan The antitumor β-glucan from the fruit body of Grifola frondosa ( Maitake ) was resolved by DEAE ­Sephadex chromatography into neutral and acidic components,...

[#1986-02] Effect of Glucans on the Anti...

Chem. Pharm. Bull. 1986; 34(5): 2149-54. Effect of Glucans on the Antitumor Activity of Grifolan Ohno, N(a), Hayashi, M (a), Iino, K (a), Suzuki, I (a), Oikawa, S (b), Sato, K (b), Suzuki, Y (b), Yadomae , T (a) a)Tokyo College of Pharmacy, b) Nippon Beet Sugar Mfg. Co. Ltd. The effects of antitumor active [β-1,3-glucan(grifolan)] and inactive [β-1,6-(islandican), α-1,4-(starch)]glucans in mice were compared. β-1,6-Glucan enhanced the antitumor activity of grifolan, whereas  α-1,4-glucan showed a suppressive effect on the antitumor activity of grifolan. When these...

[#1986-01] Fractionation and Characteriz...

Fractionation and Characterization of Antitumor Polysaccharides from Maitake, Grifola frondosa Mizuno, T, Ohsawa, K, Hagiwara, N, Kuboyama, R Dept. of Agricultural Chemistry, Faculty of Agriculture, Shizuoka University Three groups of polysaccharides from the edible mushroom, “Maitake”, the cultured fruiting body of Grifola frondosa, were extracted with hot water, 3% NH4-oxalate (100o C), and 5% sodium hydroxide solution (30o C ) in this order. The three fractions, FI, FII, and FIII, were divided into several sub-fractions using various chromatagraphic...

[#1985-07] Structural Characterization o...

Structural Characterization of a Neutral Antitumor Beta-D-Glucan Extracted with Hot Sodium Hydroxide from Cultured Fruit Bodies of Grifola frondosa Iino, K(a), Ohno, N(a), Suzuki, I(a), Miyazaki, T(a), Yadomae, T(a), Oikawa, S(b), Sata, K(b) a) Laboratory of Immunopharmacology of Microbial Proudcts, Tokyo College of Pharmacy, b) Nippon Beet Sugar Mfg. Co. Ltd. The structural characterization of an antitumor beta-D-glucan (grifolan-7N) obtained from the hot sodium hydroxide extract of Grifola frondosa ( maitake ), is described. Grifolan-7N, purified by digestion with...