[#1986-08] Maitake and other mushrooms: ...

Carbon-13 Nuclear Magnetic Resonance Spectroscopy of Some Edible Mushrooms NAOHITO OHNO, IWAO SUZUKI, and TOSHIRO YADOMAE* Tokyo College of Pharmacy, Horinouchi, Hachioji, Tokyo 192-03, Japan In this paper, carbon-13 nuclear magnetic resonance (13C-NMR) spectra of some edible Mushrooms, Grifola frondosa ( maitake ), Lentinus edodes, Flammulina velutipes, Pholiota nameko, Agaricus bisporus, Lyophyllum cinerascens, Lyophyllum aggregatum, and Peziza vesiculosa, as aqueous suspensions were measured and the glucan compositions in these mushrooms were compared. The “C-NMR...

[#1986-07] Characterization of the Antit...

Characterization of the Antitumor Glucan Obtained from Liquid-Cultured Grifola frondosa Ohno, N (a), Adachi, Y (a), Suzuki, I (a), Sato, K (b), Oikawa, S (b), Yadomae, T(a) a) Tokyo College of Pharmacy, b) Nippon Beet Sugar Mfg. Co. Ltd. Chemical characterization of the extracts, and purification and structural characterization of an antitumor glucan obtained from liquid-cultured mycelium of Grifola frondosa ( maitake ) are described in this paper. The mycelium (13 g dry weight/l culture) was extracted successively with hot water (LMHW, 14.9 g/10g mycelium), cold alkali...

[#1986-06] Two Different Conformations o...

Two Different Conformations of Antitumor Glucans Obtained from Grifola frondosa Ohno, N (a), Adachi, A (a), Suzuki, A (a), Oikawa, S (b), Sato, K (b), Suzuki, Y (b), Ohsawa, M (b), Yadomae, T (a) a) Tokyo College of Pharmacy, b) Nippon Beet Sugar Mfg. Co. Ltd. Several antitumor glucan fractions obtained by various extraction procedures from Grifola frondosa ( maitake ) were compared by solution and solid (cross polarization, magic angle spinning (CP/MAS)) carbon-13 nuclear magnetic resonance (13C-NMR) spectroscopy. The fractions prepared under milder conditions, i.e. hot...

[#1986-05] Antitumor activity of a β-1,3...

Antitumor activity of a β-1,3-glucan obtained from liquid cultured mycelium of Grifola frondosa Ohno N, Adachi Y, Suzuki I, Oikawa S, Sato K, Ohsawa M, Yadomae T. Tokyo College of Pharmacy The antitumor activity of a branched β-1,3-glucan “grifolan LE” purified from liquid cultures of Grifola frondosa ( maitake ) (Ohno et al. Chem. Pharm. Bull., 34, 1709-1715, 1986) was examined on an allogeneic murine tumor system. By intraperitoneal (i.p.) administration (100-200 μg/mouse/d X 5) at days 1 to 9 from the tumor transplantation, grifolan LE showed marked...

[#1986-04] Effect of Activation or Block...

Effect of Activation or Blockade of the Phagocytic System on the Antitumor Activity of Grifolan NAOHITO OHNO,a MAMI HAYASHI,a IWAO SUZUKI,a SHOZO OIKAWA,b KICHIRO SATO,b YOSHIYUKI Suzuiu,b and TOSHIRO YADOMAE*‘ a Tokyo College of Pharmacy,a and Nippon Beet Sugar Mfg., Co., Ltd.,b The effect of activation or blockade of the phagocytic system on the antitumor activity of grifolan from Maitake was investigated. The activity of grifolan was abrogated by treatment of mice with a blocker of the phagocytic system, i.e., carrageenan, colloidal carbon, or trypan blue. The...

[#1986-03] Fractionation of Acidic Antit...

Fractionation of Acidic Antitumor β-Glucan of Grifola frondosa by Anion-Exchange Chromatography Using Urea Solutions of Low and High Ionic Strengths NAOHITO OHNO,a KAZUYOSHI IINO,a SHOZO OEKAWA,b KICHIRO SATO,b MASUMI OHSAWA,b and TOSHIRO YADOMAE*‘ a Tokyo College of Pharmacy,’ Horinouchi, Hachioji, Tokyo 192-03, Japan and Nippon Beet Sugar Mfg. Co., Ltd.,’ Kyobashi, Chuo-ku, Tokyo 104, Japan The antitumor β-glucan from the fruit body of Grifola frondosa ( Maitake ) was resolved by DEAE ­Sephadex chromatography into neutral and acidic components,...