[#1985-02] Purification and Structural C...

Purification and Structural Characterization of an Antitumor β-1,3-glucan Isolated from Hot Water Extract of the Fruit Body of Cultured Grifola frondosa Ohno, N(a), Suzuki, I(a), Sato, K(b), Oikawa, S(b), Miyazaki, T(a), Yadomae, T(a) (a) Tokyo College of Pharmacy, (b) Nippon Beet Sugar Mfg. Co, Ltd. Polysaccharides in the hot water extract of the fruit bodies of G. frondosa ( maitake ) contain α-1,4-, α-1,6-, β-1,6- and β-1,3-glucosidic linkages, and include an antitumor-active substance assumed to be a β-1,3-glucan (N. Ohno et al Chem. Pharm. Bull. 32, 1142...

[#1985-01] Structure-Function Relationsh...

Structure-Function Relationship of Antitumor β-1,3-Glucan Obtained from Matted Mycelium of Cultured Grifola frondosa Iino, K(a), Ohno, N(a), Suzuki, I(a), Sata, K(b), Oikawa, S(b), Yadomae, T(a) a) Tokyo College of Pharmacy, b) Nippon Beet Sugar Mfg. Co. Ltd The structure-function relationship of the antitumor 6-branched β-1,3-glucan (grifolan NMF-5N) extracted from the matted mycelium of Grifola frondosa ( maitake ) was examined by using various chemical modification procedures (periodate oxidation, Smith degradation, and enzymic hydrolysis). The antitumor activity of...

[#1984-02] Antitumor activity of a polys...

Antitumor activity of a polysaccharide fraction extracted from cultured fruiting bodies of Grifola frondosa Suzuki I, Itani T, Ohno N, Oikawa S, Sato K, Miyazaki T, Yadomae T. Tokyo College of Pharmacy Antitumor activity of a polysaccharide fraction (GF-1) extracted from cultured fruiting bodies of a fungus, Grifola frondosa ( maitake ), was examined on allogeneic and syngeneic tumors in mice. GF-1 had a marked inhibitory activity against the growth of subcutaneously (s.c.) inoculated Sarcoma 180 by the intraperitoneally (i.p.) injection at 0.5-5.0 mg/mouse for 10...

[#1984-01] Antitumor Activity and Struct...

Antitumor Activity and Structural Characterization of Glucans Extracted from Cultured Fruit Bodies of Grifola frondosa Ohno N(a), Suzuki, I(a), Oikawa, S(b), Sato, K(b), Miyazaki, T(a), Yadomae, T(a) (a) Tokyo College of Pharmacy, (b) Nippon Beet Sugar Mfg. Co., Ltd., Tokyo, Japan Polysaccharides in the cultured fruit body of Grifola frondosa ( maitake ) were extracted successively with hot water, aqueous zinc chloride, and cold and hot aqueous sodium hydroxide containing urea. The antitumor effect and structural features of the extracted compounds were examined. The above...

[#1983-02] Structural Analysis of the Be...

Structural Analysis of the Beta-D-Glucan Extracted with Aqueous Zinc Chloride from the Fruit Body of Grifola frondosa Kato, K, Inagaki, T, Shigagaki, H, Yamauchi, T, Okuda, K, Sano, T, Ueno, Y Department of Agricultural Chemistry, Gifu University The defatted fruit-body of G. frondosa ( maitake ) was successively extracted with water and 10% aqueous zinc chloride in an autoclave. The polysaccharide (F4) extracted with the zinc chloride solution had [alpha]D +7.9deg(c 0.3, 4% sodium hydroxide) and was composed of beta-D-glucose. From the results of methylation study, Smith...

[#1983-01] Maitake: Kato, K et al ‘...

Structural Analysis of the Alkali-Soluble β-D-Glucan from the Fruit Body of Grifola frondosa Kato, K., Inagaki, T., Teranishi, T., Yamauchi, R., Okuda, K., Sano, T., Ueno, Y. Department of Agricultural Chemistry, Gifu University The polysaccharide was extracted from the fruit body of Maitake (G. frondosa) with 10% sodium hydroxide. It was further fractionated, to afford F7 (precipated by neutralization of the extract) and F8 (unprecipitated). F7 and F8 were found to be homogeneous β-D-glucans and they had [α]D +10deg and +3.3deg (c0.3, 4% sodium hydroxide),...