The pilot study evaluated the effects of a 4-week administration of H. erinaceus (Amyloban 3399) on female undergraduate students who were likely to have a high incidence of sleep problems. We assessed the changes in asleep quality and subjective well-being with the GHQ-28 and PSQI (Pittsburgh Sleep Quality Index). Furthermore, we examined the level of salivary free-MHPG after awakening, which is generally regarded as an accurate index of chronic stress and depressive symptoms and reflects sympathetic nervous system activity. Thus, we were able to conduct a comprehensive analysis of the effect of Amyloban 3399 on sleep quality and subjective well-being of female students.