[#1983-02] Structural Analysis of the Beta-D-Glucan Extracted with Aqueous Zinc Chloride from the Fruit Body of Grifola frondosa, K. Kato et al.

Structural Analysis of the Beta-D-Glucan Extracted with Aqueous Zinc Chloride from the Fruit Body of Grifola frondosa

Kato, K, Inagaki, T, Shigagaki, H, Yamauchi, T, Okuda, K, Sano, T, Ueno, Y

Department of Agricultural Chemistry, Gifu University

The defatted fruit-body of G. frondosa ( maitake ) was successively extracted with water and 10% aqueous zinc chloride in an autoclave. The polysaccharide (F4) extracted with the zinc chloride solution had [alpha]D +7.9deg(c 0.3, 4% sodium hydroxide) and was composed of beta-D-glucose. From the results of methylation study, Smith degradation and enzymic hydrolysis it was concluded that F4 is a polysaccharide composed of a backbone of beta-(1->3)-linked D-glucopyranosyl residues, and possessed of a single beta-D-glucopyranosyl group joined through O-6 of every third D-glucopyranosyl residue of the backbone. The molecular weight was determined by gel filtration to be 140,000.