[#1987-01] The Chemical Structure of an Antitumor Polysaccharide in Fruit Bodies of Grifola frondosa (Maitake), Hiroaki Nanba et al.

The Chemical Structure of an Antitumor Polysaccharide in Fruit Bodies of Grifola frondosa (Maitake)

Nanba, H, Hamaguchi, A, Kuroda, H

Laboratory of Microbiology, Kobe Women’s College of Pharmacy

A polysaccharide was extracted from fruit bodies of Grifola frondosa ( maitake ), and the chemical structure and antitumor activity was studied. The extracted polysaccharide could be hydrolyzed by beta-glucanase into glucose, indicating it to be a beta-glucan. The sample gave methyl 2,3,4,6-tetra-O-methyl 2,4,6-tri-O-, methyl2,3,4-tri-O- and methyl 2,4-di-O-methylglucoside in the molar ratio of 4:2:1:4 on methylation. In the carbon-13 nuclear magnetic resonance spectrum, the signals of C-6′ (related to (1-6) bonding) and C-3′ (related to (1-3) bonding) were observed in addition to those of free C-6 and C-3. These results indicate that the major chain is made up of β-1,6-linked glucose residues with branches of beta-1,3-linked glucose. This glucan inhibited the growth of Sarcoma 180 tumor in ICR mice.