[#1987-03] Effect of Grifolan on the Ascites Form of Sarcoma 180, Naohito Ohno et al.

NAOHITO OHNO,^b YOSHIYUKI SUZUKI,^b KICHIRO SATO,^bSHOZO OIKAWA,^b and TOSHIRO YADOMAO’^b

Tokyo College of Pharmacy,’ Horinouchi, Hachioji, Tokyo 192-03,
Japan and Nippon Beet Sugar Mfg. Co., Ltd.,’
Kyobashi, Chuo-ku, Tokyo 104, Japan

The antitumor activity of a (1–>3)-β-D-glucan, grifolan from Maitake , which was obtained from a fungus, Grifola frondosa, on the ascites form of Sarcoma 180 in ICR mice was examined. Grifolan was effective on the ascites tumor when it was administered before tumor transplantation (days —7­- I) as a single dose of 2 mg/mouse by i.p. injection, but administration after the tumor transplantation was ineffective. The antitumor activity was transferred to untreated mice by injecting the peritoneal exudate cells obtained from grifolan-treated mice. The antitumor activity of grifolan was augmented by vaccination of mice with live sarcoma 180 cells (s.c. injection).