[#1987-06] ANTITUMOR EFFECT OF POLYSACCHARIDE GRIFOLAN NMF-5N ON SYNGENEIC TUMOR IN MICE, I. Suzuki et al.
ANTITUMOR EFFECT OF POLYSACCHARIDE GRIFOLAN NMF-5N ON SYNGENEIC TUMOR IN MICE
IWAO SUZUKI,* TAKAHIRO TAKEYAMA,* NAOHITO OHNO,* SHOZO OIKAWA,** KICHIRO SATO,** YOSHIYUKI SUZUKI,** AND TOSHIRO YADOMAE*
Tokyo College of Pharmacy, * Horinouchi, Hachioji, Tokyo, 192-03, Japan and Nippon Beet Sugar Mfg., Co. Ltd., ** Chuo-ku, Tokyo, 104, Japan
Antitumor activity of grifolan (from maitake ) NMF-5N, a β-1,3-glucan obtained from mycelia of Grifola frondosa, was examined. Grifolan NMF-5N showed antitumor activities in allogeneic and syngeneic murine tumor systems. In the allogeneic tumor system, a potent antitumor activity over 95% was observed against the solid form of sarcoma 180 when grifolan NMF-5N was injected intraperitoneally (i.p.) at 25 —200 µg/mouse daily for 10 successive days. In the syngeneic tumor systems, significant antitumor activities were observed against Meth A fibrosarcoma and MM 46 carcinoma by injection at 100 ug/mouse daily for 5 successive days, especially i.p. injection at day 7-11, when the tumor cells were inoculated subcutaneously (s.c.) on day 0. Moreover, when grifolan NMF-5N was injected i.p. every other week, significant antitumor activity was also observed. In addition, a single treatment with grifolan NMF-5N at 500 ug/mouse showed antitumor activities. Grifolan NMF-5N exhibited antitumor activities against these two syngeneic tumors by intraveneous (i.v.) injection. However, a marked inhibitory activity was observed by intratumorous (i.t.) injection against Meth A fibrosarcoma but not against MM46 carcinoma. These results suggest that antitumor activities of grifolan NMF-5N in murine syngeneic tumor systems depend on not only dosage but also injection routes and timing.
