[#1994-06] Monoclonal antibody to proteoglycan derived from Grifola frondosa (Maitake), Akio Hirata et al.

Monoclonal antibody to proteoglycan derived from Grifola frondosa (Maitake)

Hirata A, Adachi Y, Itoh W, Komoda M, Tabata K, Sugawara I.

Research Laboratory, Taito Co., Ltd.

A murine monoclonal antibody (MAb) was prepared by immunizing BALB/c mice with a proteoglycan fraction derived from Grifola frondosa ( Maitake  mushroom), followed by the hybridization of spleen cells with mouse myeloma cells. The MAb (subclass; Ig G2b), designated MPG2, reacted with schizophyllan (SPG), curdlan, scleroglucan, laminarin and lentinan, but not with dextran, pullulan, mannan and xylan. Immunohistochemistry (ABC-GO method) showed that MAb MPG2 reacted with lysosomal proteoglycan and (1->6)-β-branched laminaritriose taken up by rabbit peritoneal macrophages. These results suggest that this MAb may recognize mainly (1->3)-β-D-glucan, and may be useful for determining the immunological properties of Grifola frondosa-derived proteoglycan.