[#2004-11] Purification and Characterization of a Novel Prolyl Aminopeptidase from Maitake (Grifola frondosa), Kazuyuki Niwatashi et al.
Purification and Characterization of a Novel Prolyl Aminopeptidase from Maitake (Grifola frondosa)
Kazuyuki HIWATASHI,1 Kazuyuki HoRI,I Keitaro TAKAHASHI,’ Akira KAGAYA,2 Shunzo INouE,2 Toshihiro SUGIYAMA,3 and Saori TAKAHASHIl‘t
‘Department of Bioengineering, Akita Research Institute of Food and Brewing,2Akita Jujo Chemicals Co., Ltd.3Department of Biochemistry, Akita University School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan
We have found a novel prolyl aminopeptidase in Maitake (Grifola frondosa). The enzyme was purified by DEAE-Sepharose CL-6B, Butyl-Toyopearl, Sephacryl S-100, and Mono-Q column chromatographies. The purified enzyme exists as a dimer and gives high activity toward L-proline-p-nitroanilide. The enzyme was strongly inhibited by p-chloromercuribenzoic acid and iodoacetic acid and markedly inhibited by phenylmethylsulfonyl fluoride and arphamenin A.
