[#2005-05] Characterization, Occurrence, and Molecular Cloning of a Lectin from Gnfola frondosa: Jacalin-Related Lectin of Fungal Origin, Yoshiho Nagata et al.
Characterization, Occurrence, and Molecular Cloning of a Lectin from Gnfola frondosa: Jacalin-Related Lectin of Fungal Origin
Yoshiho NAGATA,l‘t Masako YAMASHITA,’ Hiroaki HONDA,’ JUT1k0 AKABANE,’ Koichi UEHARA,’ Akihiro SArro,1 Futoshi SumtsA,1 Kozo NISHIBORI,2 and Yasuo OODAIRA2
‘Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University, 2Section of Research and Development, Yukiguni-Maitake Co., Ltd.
A lectin named GFL was isolated from the fruiting body of the basidiomycete mushroom Grifola frondosa, which belongs to Aphyllophorales. The lectin had a molecular mass of 24 kDa on SDS—PAGE. The hemagglutinating activity of GFL was not inhibited by any monosaccharide, and inhibited only by porcine stomach mucin so far as tested. The occurrence of GFL was studied at three stages during fruiting body formation. The largest quantity of hemagglutinating activity was found in the fruiting body, and lesser amounts in the mycelial mat and the primordium. The 24-kDa band of GFL was found at all three stages, and the band-intensity corresponded to the level of activity in each sample. By cloning and sequencing the GFL-cDNA, the primary structure of this lectin was determined. GFL is composed of 181 amino acids, having no signal peptide. The amino acid sequence was found to be homologous to those of so-called jacalin-related plant lectins, suggesting that GFL is the first example of a jacalin-related lectin of fungal origin.
