[#2006-03] Maitake Miyake, Naomi et al. Low molecular weight maitake MD-Fraction (Klasma-MD) hydrolyzed with endo-β-1,6-glucanase of Trichoderma harzianum induces antitumor activities

Low molecular weight maitake MD-Fraction (Klasma-MD) hydrolyzed with endo-β-1,6-glucanase of Trichoderma harzianum induces antitumor activities

Miyake N, Masuda Y, Murata Y, Inoue M, Matuda K, Okamoto K, Nanba H, Kodama N

Department of Microbial Chemistry, Kobe Pharmaceutical University

We previously reported that MD-Fraction (Klasma-MD), a specific-structured β-glucan extracted from fruit bodies of maitake mushrooms (Grifola frondosa), can activate cellular immune systems and express antitumor effects. The molecular weight of the MD-Fraction ranged from 1,200,000 to 2,000,000. The molecular weight of the products of the MD-Fraction obtained after hydrolysis by endo-β-1,6-glucanase from Tricoderma harzianum was reduced to 250,000 in terms of dextran, and these products inhibited MM-46 carcinoma in C3H/HeJ mice by 67.9% after intraperitoneal injection. In addition, interleukin-12 and interferon-γ values were increased 1.4 fold and 2.1 fold, respectively, compared with control values. From these results, low molecular weight MD-Fraction (L-MD-Fraction) maintains an immunological active site.