[#2006-04] Macrophage J774.1 cell is activated by MZ-Fraction (Klasma-MZ) polysaccharide in Grifola frondosa, Yuki Masuda et al.

Macrophage J774.1 cell is activated by MZ-Fraction (Klasma-MZ) polysaccharide in Grifola frondosa

Masuda Y, Kodama N, Nanba H

Department of Microbial Chemistry, Kobe Pharmaceutical University

We previously reported that the (β-1,3)-branched (β-1,6)-glucan, MD-Fraction extracted from the fruit body of the edible maitake mushroom (Grifola frondosa) exhibits an antitumor effect. In this study, we separated a new polysaccharide from maitake, which we named Maitake MZ-Fraction (Klasma-MZ). MZ-Fraction is a β-glucan with a low molecular weight of about 20 000, which has a β-1,3- and a β-1,6-bond. MZ-Fraction enhanced TNF-α and IL-12 productivity and the antigen presentation of murine macrophage cell line J774.1 in vitro. Furthermore, MZ-Fraction showed antitumor activity similar to MD-Fraction in vivo.