[#2010-14] Influence of Culinary-Medicinal Maitake Mushroom, Grifola frondosa (Dicks.: Fr.) S.F. Gray (Aphyllophoromycetideae) Polysaccharides on Gene Expression in Jurkat T Lymphocytes, Natasa Radic et al.
Influence of Culinary-Medicinal Maitake Mushroom, Grifola frondosa (Dicks.: Fr.) S.F. Gray (Aphyllophoromycetideae) Polysaccharides on Gene Expression in Jurkat T Lymphocytes
Natasa Radic,1* Zala Jevnikar,1 Natasa Obermajer,1,2 Jernej Kristl,1 Janko Kos,1,2 Franc Pohleven,3 & Borut Strukelj1,2
1Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Slovenia; 2Department of Biotechnology, Jozef Stefan Institute, Slovenia; 3Biotechnical Faculty, University of Ljubljana, Slovenia
ABSTRACT:
The fruiting body of the culinary-medicinal Maitake mushroom Grifola frondosa contains β-glucans that have immunomodulatory effects and potential uses as adjuncts to cancer therapy. Numerous clinical trials are being conducted to assess the benefits of using commercial preparations containing G. frondosa extracts in cancer treatment. We tested a commercially available product containing polysaccharides from the G. frondosa (CGFP) and studied their effect on gene expression in Jurkat T cells. An mRNA differential display was performed to gain insight into the cell response to the substance. HAI1, hnRNP A2/B1, PKC iota, KLRC1, and genes of the ubiquitin system were identified as possible regulators in the mechanism of tumor-suppressor action of CGFP. Real-time polymerase chain reaction (qPCR) was used to investigate the effect of CGFP on cytokine expression in Jurkat T cells. The qPCR results showed that the expression of the gene for IL-5 is increased.
