[#2008-01] Chaga mushroom (Inonotus obliquus) induces G0/G1 arrest and apoptosis in human hepatoma HepG2 cells, Myung-Ja Youn et al.

Myung-Ja Youn, Jin-Kyung Kim, Seong-Yeol Park, Yunha Kim, Se-Jin Kim, Hong Seob So, Raekil Park, Vestibulocochlear Research Center, Wonkwang University School of Medicine, Jeonbuk, Korea

Ki-Young Kim, Ming-Xun Cui,College of Human Environmental Science, Wonkwang University, Jeonbuk, Korea

Jin Seok Lee, Kyu Yun Chai, Department of Bionanochemistry, College of Natural Science, Wonkwang University, Jeonbuk, Korea

Abstract

AIM: To investigate the anti-proliferative and apoptotic effects of Chaga mushroom (Inonotus obliquus) water extract on human hepatoma cell lines, HepG2 and Hep3B cells.

METHODS: The cytotoxicity of Chaga extract was screened by 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. Morphological observation, flow cytometry analysis, Western blot were employed to elucidate the cytotoxic mechanism of Chaga extract.

CONCLUSION: Chaga mushroom may provide a new therapeutic option, as a potential anticancer agent, in the treatment of hepatoma.