Purification and Characterization of an Aminopeptidase from the Edible Basidiomycete Grifola frondosa ( Maitake ) Toshikazu NISHIWAKI and Kiyoshi HAYASHI National Food Research Institute, Ministry of Agriculture, Forestry, and Fisheries, Ibaraki Japan An aminopeptidase was purified 178-fold from an extract of Maitake ( Grifola frondosa ) by ammonium sulfate precipitation and a series of column chromatographies on phenyl-Toyopearl, Sephadex G-25, and Mono-Q. The molecular mass of the enzyme was estimated to be 27 kDa and 30 kDa...
read morePurification and Characterization of Mushroom Proteases. 4. Maitake, Grifola frondosa Emi KENJO, Yoko YAMAGUCHI and Kosaku NODA Laboratory of Biochemistry, Department of Nutrition and Health Science, Faculty of Human Environmental Science, Fukuoka Women’s University, Japan Recently, proteolytic enzymes are becoming increasingly important especially in the food industries.” Today the major uses of proteases occur in production and processing of diverse products such as cheese,” 6) baked goods,7‘ beer,9‘...
read moreRelationship between Solubility of Grifolan, a Fungal 1,3-β-D-Glucan, and Production of Tumor Necrosis Factor by Macrophages in Vitro. Ishibashi K, Miura NN, Adachi Y, Ohno N, Yadomae T. Laboratory for Immunopharmacology for Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science Grifolan, GRN, is a fungal antitumor β-glucan isolated from Grifola frondosa ( maitake ). Various studies suggested that the underlying mechanism of the antitumor activity of GRN is strongly related to immune modulation. In the previous...
read moreAddition of Maitake D-fraction reduces the effective dosage of vancomycin for the treatment of Listeria-infected mice Kodama N, Yamada M, Nanba H. Department of Microbial Chemistry, Kobe Pharmaceutical University Maitake D-fraction, β1,6-glucan having β,3-branches, has been reported to activate the immune system of the host. To elucidate whether the D-fraction can reduce the clinical effective dosage of antibiotics in the treatment of opportunistic bacterial infection, we examined the effects of D-fraction on the treatment of Listeria...
read moreMaitake Extract and Their Therapeutic Potential — A Review Mark Mayell Abstract Maiitake (Grifola frondosa) is the Japanese name for an edible fungus with a large fruiting body characterized by overlapping caps. It is a premier culinary as well as medicinal mushroom, Maitake is increasingly being recognized as a potent source of polysaccharide compounds with dramatic health-promoting potential. The most recent development is the MD-fraction, a proprietary maitake extract its Japanese inventors consider to be a notable advance upon the...
read moreEffects of maitake (Grifola frondosa) D-Fraction on the carcinoma angiogenesis Matsui K, Kodama N, Nanba H. Department of Microbial chemistry, Kobe Pharmaceutical University We have reported that D-Fraction extracted from maitake (Grifola frondosa), activates immune competent cells, and indicates anti-tumor activities. The D-Fraction was observed to induce angiogenesis in vivo and to enhance the proliferation capability and migration capability of human vascular endothelial cell in vitro. The D-Fraction also increased plasma vascular...
read moreMedicinal Value of the Genus Tremella Pers. (Heterobasidiomycetes) (Review) Sergey V Reshetnikov,1‘3 Solomon P Wasser,1‘2 Eviatar Nevo,2 Ina Duckman,3 and Katherina Tsukor3 1N. G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Ukraine; 2 lnstitute of Evolution, University of Haifa, Mt. Carmel, Israel; 3 Med Myco Ltd., Institute of Evolution, University of Haifa, Israel ABSTRACT: Jelly mushrooms from the genus Tremella have been known in Oriental medicine for hundreds of years as beneficial for...
read moreStructures of New Ceramides from the Fruit Bodies of Grifola frondosa Yasunori YAOITA, Takaaki ISHIZUKA, Rie KAKUDA, Koichi MACHIDA, and Masao KIKUCHI* ToKyo Pharmaceutical University, Japan. Four new phytosphingosine-type ceramides, (25,35,4R)-2-[(2'R)-2'-hydroxydocosanoylamino1-1,3,4-octadecanetriol (1), (2S,3S,4R)-2-[(2'R)-2'-hydroxytricosanoylamino]-1,3,4-octadecanetriol (2), (2S,3S,4R)-2-[(2'R)-2'- hydroxypentacosanoylamino]-1,3,4-octadecanetriol (3) and (2S,3S,4R)-2-[(2'R)-2'-hydroxyhexacosanoylamino]- 1,3,4-octadecanetriol (4), have...
read moreAngiotensin I-Converting Enzyme Inhibitory Peptides from Pepsin Digest of Maitake (Grifola frondosa) Ohtsuru, M(a), Horio, H(b), Masui, H(c) a) Department of Food Science and Nutrition, Mukogawa University, b) Department of Food Science and Nutrition, Nishikyushu University, c) Department of Dietary Life and Food Science, Mukogawa Women’s University Junior College Division Three peptides which inhibit angiotension I-converting enzyme were isolated from maitake (Grifola frondosa) water extract digested by pepsin. Among them,...
read moreEffects of Maitake (Grifola frondosa) glucan in HIV-infected Patients Nanba, H(a), Kodama, N(a), Schar, D(b), Turner, D(b) a) Department of Microbial Chemistry, Kobe Pharmaceutical University, b) Herbal Clinical Research, London The effects of MD-Fraction, a β-glucan extracted from Maitake mushroom (Grifola frondosa), on the health status of individuals suffering from HIV infection were evaluated in a long-term trial. The HIV status of the 35 respondents who participated in the study was followed by monitoring CD4+ cell counts, viral load...
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