Fractionation and Antitumor Activity of Polysaccharides from Grifola frondosa Mycelium Zhuang, C, Mizuno, T, Ito, H, Shimura, K, Sumiya, T, Kawade, M The United Graduate School of Agricultural Sciences, Gifu University We developed a method for the fractionation and purification of antitumor polysaccharides, considered to be a type of immunopotentiator or BRM (biological response modifier), from the mycelium of liquid cultured Grifola frondosa ( maitake ). The active polysaccharide fractions that showed higher antitumor activity were...
read moreAnti-diabetic activity present in the fruit body of Grifola frondosa (Maitake) Kubo K(a), Aoki H(b), Nanba H(c) a) Yukiguni Maitake Co., Ltd., b) Aoki Clinic, c) Department of Microbial Chemistry, Kobe Pharmaceutical University The fruit body of Grifola frondosa ( maitake ), Basidiomycetes was confirmed to contain substances with anti-diabetic activity. When 1 g/d of powdered fruit body of maitake was given orally to a genetically diabetic mouse (KK-Aγ), blood glucose reduction was observed, in contrast to the control group in which...
read moreEFFECTS OF A COPPER-BINDING PEPTIDE FROM THE MUSHROOM GRIFOLA FRONDOSA ON INTESTINAL COPPER ABSORPTION Iwao Shimaoka, M.S.1*, Sachiko Ishiyama, B.S.2, Kohsuke Nishino, Ph.D.2, Yoshinori Itokawa, Ph.D.’ ‘Department of Environmental Medicine. Graduate Schoolof Medicine. KyotoUniversity, Kyoto, Japan2Department of Home Economics, Dohshisha Women’s College,Kyoto, Japan ABSTRACT The effect of a copper-binding peptide, purified previously from the fruit bodies of the mushroom, Grifola frondosa ( maitake ) , on copper absorption...
read moreMonoclonal antibody to proteoglycan derived from Grifola frondosa (Maitake) Hirata A, Adachi Y, Itoh W, Komoda M, Tabata K, Sugawara I. Research Laboratory, Taito Co., Ltd. A murine monoclonal antibody (MAb) was prepared by immunizing BALB/c mice with a proteoglycan fraction derived from Grifola frondosa ( Maitake mushroom), followed by the hybridization of spleen cells with mouse myeloma cells. The MAb (subclass; Ig G2b), designated MPG2, reacted with schizophyllan (SPG), curdlan, scleroglucan, laminarin and lentinan, but not with dextran,...
read moreMaitake – The King of Mushrooms Anthony J. Cichoke, MA, DC, DACBN For over 3,000 years, Traditional Chinese Medicine (TCM) has enjoyed an illustrious history. Today, TCM and Western medicine function side-by-side in Japan, as can be witnessed by the frequent use of maitake mushrooms to deal with conditions which, in the U.S., are usually treated with drugs. In Japan, maitake (pronounced mytah-keh) mushrooms are highly prized both as food and medicine. People with serious degenerative illnesses travel long distances in search...
read moreEnhancement of cytokine production by macrophages stimulated with (1->3)-beta-D-glucan, grifolan (GRN), isolated from Grifola frondosa Adachi Y, Okazaki M, Ohno N, Yadomae T. Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science The ability of grifolan (GRN, maitake derived ), a purified fungal (1->3)-β-D-glucan, to induce various cytokines from macrophages was examined in vitro. Interleukin-6 (IL-6) activity in supernatants from the culture of macrophage cell line, RAW264.7 was dependent...
read morePreparation and Antigen Specificity of an Anti-(1->3)-β-D-glucan Antibody Yoshiyuki ADACHI, Naohito OHNO, and Toshiro YADOMAE Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, Tokyo, Japan. Antibody for (1-6)-branched (1-3)-β-D-glucan was prepared using rodents. An antitumor (1-6)-β-monoglucosyl branched (1-3)-β-D-glucan (GRN: grifolan from maitake ) was conjugated with bovine serum albumin and used as an immunogen. The antibody titer in serum was determined by ELISA using biotin-conjugated GRN....
read moreErinacines A, B and C, strong stimulators of nerve growth factor (NGF)-synthesis, from the mycelia of Hericium erinaceum. Hirokazu Kawagishi*, Atsushi Shimada, Ryoko Shiraia, Kenji Okamotob, Fumihiro Ojimab, Hideki Sakamotob, Yukio Ishiguro, and Shoei Furukawa Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422, Japan. Research Institute, Kagome Co., Ltd., 17 Nishitomiyama, Nishinasuno-cho, Tochigi 329-27, Japan. cDepartment of Molecular Biology, Gifu Pharmaceutical University, 5-6-1...
read moreTHE REVISED HASEGAWA’S DEMENTIA SCALE(HDS-R) — EVALUATION OF ITS USEFULNESS AS A SCREENING TEST FOR DEMENTIA YUKIMICHI IMAI & KAZUO HASEGAWA Summary Revised Hasegawa’s dementia scale (HDS-R), consisting of 9 simple questions with a maximum score of 30, was examined in its usefulness for screening age-associated dementia in a total of 157 subjects: 95 demented patients and 62 non-demented persons. The two groups were age-matched. Cronbach’s coefficient alpha was as high as 0.90 in HDS-R. In addition, the coefficient...
read moreβ-Glucans Inhibitory Effect of Beta-Glucans on Zymosan-Mediated Hydrogen Peroxide Production by Murine Peritoneal Macrophages in Vitro Adachi, Y, Ohno, N, Yadomae, T Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy Effects of the pretreatment of murine peritoneal macrophages with several polysaccharides on the production of H2O2 induced with unopsonized zymosan were examined. Pretreatment with most of (1->3)-β-D-glucans for 6h at 37deg C inhibited the zymosan-mediated H2O2 production by macrophages....
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