[#1998-05] Purification and Characteriza...

Purification and Characterization of a Trehalose Synthase from the Basidiomycete Grifola frondosa ( Maitake ) KOKI SAITO,1 TOSHIYA KASE,1 EIICHI TAKAHASHI,1 EISAKU TAKAHASHI,1 AND SUEHARU HORINOUCHI2* Nishiki Research Laboratories, Kureha Chemical Industry Co., Ltd.,Fukushima, Japan,1 and Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo, Japan2 A trehalose synthase (TSase) that catalyzes the synthesis of trehalose from D-glucose and α-D-glucose 1-phosphate (α-D-glucose 1-P) was detected in a basidiomycete,...

[#1998-04] Modification of cellular immu...

Modification of cellular immune responses in experimental autoimmune hepatitis in mice by maitake (Grifola frondosa) Keiko Kubo and Hiroaki Nanba Department of Microbial Chemistry,KobePharmaceuticalUniversity, ,Japan   Immune response to liver-specific lipoprotein (LSP) is involved in the pathogenesis of chronic active hepatitis. Experimental hepatitis could thus be prepared in C57BL/6 mice by injection of liver-specific protein in a syngeneic liver homogenate with Freund’s complete adjuvant. In hepatitic mice treated with maitake (Grifola frondosa) fruit...

[#1998-03] Activation of by administrati...

Activation of  by administration with gel-forming (1->3)-β-D-glucan from Grifola frondosa ( Maitake ) Adachi Y, Ohno N, Yadomae T. Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science The effect of gel-forming (1->3)-β-D-glucan on the immunological activities of murine kupffer cells was examined. A branched type gel-forming (1->3)-β-D-glucan, GRN, was administered intravenously to mice. GRN associating to kupffer cells was detected by an immunohistochemical technique using anti-GRN antibody. A kinetic study of...

[#1998-02] Adjuvant Effect of Grifolan o...

Adjuvant Effect of Grifolan on Antibody Production in Mice Yoshiyuki ADACHI, Yoko SUZUKI, Naohito OHNO, and Toshiro YADOMAE* Laboratory of Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan. The effects of grifolan (GRN from maitake ), a gel-forming (1—>6)-branched (1—>3)-β-glucan, on antibody production were examined. Sera from mice that were injected with GRN and trinitrophenyl ovalbumin (TNP-OVA) in­traperitoneally showed a significantly increased level of anti-TNP IgG. However, injection of...

[#1997-08] Effects of Lentinus edodes, G...

Effects of Lentinus edodes, Grifola frondosa and Pleurotus ostreatus administration on cancer outbreak, and activities of macrophages and lymphocytes in mice treated with a carcinogen, N-butyl-N-butanolnitrosoamine Kurashige S, Akuzawa Y, Endo F. Department of Laboratory Sciences, Gunma University School of Health Sciences ICR mice were treated with a carcinogen, N-butyl-N’-butanolnitrosoamine (BBN), every day for 8 consecutive weeks and the effects of oral administration of edible mushrooms on the induction of urinary bladder carcinoma and on the activities of...

[#1997-06] Anti-hyperliposis effect of m...

Anti-hyperliposis effect of maitake fruit body (Grifola frondosa) Kubo K, Nanba H. Department of Microbial Chemistry, Kobe Pharmaceutical University Experimental rat models (5-week-old Sprague-Dawley rats) with hyperlipemia were prepared by feeding high-cholesterol feed containing sodium cholate and casein as a protein source. Dried maitake (Grifola frondosa) powder was mixed with the basic high-cholesterol feed and the serum lipids were periodically measured. Values of cholesterol, triglyceride and phospholipid in serum of rats in the maitake-feed group were suppressed by...