[#1994-06] Monoclonal antibody to proteo...

Monoclonal antibody to proteoglycan derived from Grifola frondosa (Maitake) Hirata A, Adachi Y, Itoh W, Komoda M, Tabata K, Sugawara I. Research Laboratory, Taito Co., Ltd. A murine monoclonal antibody (MAb) was prepared by immunizing BALB/c mice with a proteoglycan fraction derived from Grifola frondosa ( Maitake  mushroom), followed by the hybridization of spleen cells with mouse myeloma cells. The MAb (subclass; Ig G2b), designated MPG2, reacted with schizophyllan (SPG), curdlan, scleroglucan, laminarin and lentinan, but not with dextran, pullulan, mannan and xylan....

[#1994-05] Maitake – The King of M...

  Maitake – The King of Mushrooms Anthony J. Cichoke, MA, DC, DACBN For over 3,000 years, Traditional Chinese Medicine (TCM) has enjoyed an illustrious history. Today, TCM and Western medicine function side-by-side in Japan, as can be witnessed by the frequent use of maitake mushrooms to deal with conditions which, in the U.S., are usually treated with drugs. In Japan, maitake (pronounced my­tah-keh) mushrooms are highly prized both as food and medicine. People with serious degenerative illnesses travel long distances in search of the maitake mushroom....

[#1994-04] Enhancement of cytokine produ...

Enhancement of cytokine production by macrophages stimulated with (1->3)-beta-D-glucan, grifolan (GRN), isolated from Grifola frondosa Adachi Y, Okazaki M, Ohno N, Yadomae T. Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science The ability of grifolan (GRN, maitake derived ), a purified fungal (1->3)-β-D-glucan, to induce various cytokines from macrophages was examined in vitro. Interleukin-6 (IL-6) activity in supernatants from the culture of macrophage cell line, RAW264.7 was dependent on increasing doses of GRN....

[#1994-03] Preparation and Antigen Speci...

Preparation and Antigen Specificity of an Anti-(1->3)-β-D-glucan Antibody Yoshiyuki ADACHI, Naohito OHNO, and Toshiro YADOMAE Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, Tokyo, Japan. Antibody for (1-6)-branched (1-3)-β-D-glucan was prepared using rodents. An antitumor (1-6)-β-monoglucosyl branched (1-3)-β-D-glucan (GRN: grifolan from maitake ) was conjugated with bovine serum albumin and used as an immunogen. The antibody titer in serum was determined by ELISA using biotin-conjugated GRN. Administration of the antigen raised...

[#1993-04] Anti-Hypertensive Effect by t...

Anti-Hypertensive Effect by the King of Mushrooms Nanba, H Kobe Women’s College of Pharmacy The fungi which belong to Basidiomycetes have been used as herbal medicines for a long time. It has been found that shiitake (Lentinus edodes) and mannentake or reishi (Ganoderma lucidum) are effective against essential hypertension. The author was quite curious about the hypotensive action of Maitake (Grifola frondosa), another mushrooom belonging to basidiomycetes. In fact, the finding of this activity was a result of studying the antitumor activity of Maitake. In this...

[#1993-03] Purification of a copper bind...

Purification of a copper binding peptide from the mushroom Grifola frondosa and its effect on copper absorption Iwao Shimaoka,* Junko Kodama,* Kohsuke Nishino,t and Yoshinori Itokawa* *Department of Hygiene, Faculty of Medicine, Kyoto University; and tDepartment of Home Economics, Dohshisha Women’s College, Kyoto, Japan The present studies were undertaken to investigate the effect of a copper binding peptide on copper bioavailability. This peptide was extracted from the fruit bodies of the mushroom, Grifola frondosa ( maitake ), and purified to a single peptide...