[#1990-01] Macrophage Activation in Vitr...

Macrophage Activation in Vitro by Chemically Cross-Linked (1-3)-β-D-Glucans Yoshiyuki ADACHI,a Naohito °two,’ Masumi OHSAWA, Shozo OEKAWA and Toshiro YADOMAE Tokyo College of Pharmacy, Horinouchi, Hachioji, Tokyo 192-03, Japan and Nippon Beet Sugar Mfg. Co., Ltd., Kyobashi, Chuo-ku, Tokyo 104, Japan. Received September 11, 1989 The chemical cross-linking of soluble (1 -3)-β-D-glucans (from maitake ) having molecular weights of 21000 (CL 3 h) and 6400 (CL 6 h), and laminarin (CL LAMI), which showed negligible biological activity, by epichlorohydrin provided...

[#1989-04] Antitumor and Immunomodulatin...

Antitumor and Immunomodulating Activities of a β-Glucan Obtained from Liquid-Cultured Grifola frondosa Iwao SUZUKI,  Koichi HASHIMOTO,  Shozo OIKAWA,  Kichiro SATO  Masumi OSAWA and Toshiro YADOMAE Tokyo College of Pharmacy, Horinouchi, Hachioji, Tokyo 192-03, Japan and Nippon Beet Sugar Mfg., Co., Ltd., Chuo-ku, Tokyo 104, Japan. The effects of the β-1,3-glucan, LELFD, obtained from liquid-cultured mycelium of Grifola frondosa ( maitake ), on the growth of syngeneic tumors and immune responses in mice were examined. In Meth A or IMC solid tumor systems, LELFD...

[#1989-03] Modification of Immunostimula...

Modification of Immunostimulating Activities of Grifolan by the Treatment with (1—.3)-p-D-Glucanase Isao NONO, Naohito OHNO,* Masumi OHSAWA, Shozo OIKAWA,** and Toshiro YADOMAE* Tokyo College of Pharmacy, * Horinouchi, Hachioji, Tokyo, 192-03, Japan and Nippon Beet Sugar Mfg., Co., Ltd., ** Chuo-ku, Tokyo, 104, Japan   We have developed a method to control the effective time period of (1->3)-β-D-glucan in vivo by using (1->3)-β-D-glucanase and found that grifolan LE (GRN), from maitake ) , a (1->3)-β-D-glucan, administered intraperitoneally to the host...

[#1989-02] Modulation of Antitumor Activ...

Modulation of Antitumor Activity of Grifolan by Subsequent Administration of (1–>3)-β-D-Glucanase in Vivo Isao NONO, Naohito OHNO,* Masumi OHSAWA, Shozo OIKAWA and Toshiro YADOMAE Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy,  Horinouchi,Hachioji, Tokyo, 192-03, Japan and Nippon Beet Sugar Mfg., Co., Ltd.,  Chuo-ku, Tokyo, 104, Japan The initiation process of effector mechanism of the antitumor activity of (1–>3)-β-D-glucans has been examined in detail by applying treatment of mice with...

[#1989-01] Maitake – Physiochemic...

Maitake  - Physiochemical properties and antitumor activities of chemically modified derivatives of antitumor glucan “grifolan LE” from Grifola frondosa Adachi Y, Ohno N, Ohsawa M, Sato K, Oikawa S, Yadomae T. Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy Antitumor glucan, grifolan LE (GRN LE), from Grifola frondosa ( maitake ) was chemically modified to examine the structure-function relationship of the products. Modification by periodate, borohydride and acid hydrolysis of side chains of GRN LE did not alter properties...

[#1988-03] Distribution of Grifolan NMF-...

Distribution of Grifolan NMF-5N (I/B), a Chemically Modified Antitumor β- Glucan in Mice Takahiro TAKEYAMA, Iwao SUZUKI, Naohito OHNO,* Shozo OIKAWA, Kichiro SATO, Masumi OHSAWA,** and Toshiro YADOMAE* Tokyo College of Pharmacy, * Horinouchi, Hachioji, Tokyo, 192-03, Japan and Nippon Beet Sugar Mfg., Co., Ltd., ** Chuo-ku, Tokyo, 104, Japan   The distribution of a 3H-labeled chemically modified antitumor β-glucan, grifolan ( from maitake ) NMF-5N (I/B), in various tissues after an injection into mice was examined in order to obtain information on distribution of the...