[#1987-02] Potentiation of Host-Mediated...

Potentiation of Host-Mediated Antitumor Activity in Mice by Beta-Glucan Obtained from Grifola frondosa (Maitake) Adachi, K, Nanba, H, Kuroda, H Department of Microbiology, Kobe Women’s College of Pharmacy A polysaccharide (3-branched β-1,6-glucan MT-2) extracted from fruit bodies of Grifola frondosa ( maitake ) showed an antitumor effect against mouse syngeneic tumors (MM-46 carcinoma and IMC-carcinoma). It not only directly activates various effector cells (macrophages, Natural Killer cells, killer T-cells, etc.) to attack tumor cells, but also potentiates the...

[#1987-01] The Chemical Structure of an ...

The Chemical Structure of an Antitumor Polysaccharide in Fruit Bodies of Grifola frondosa (Maitake) Nanba, H, Hamaguchi, A, Kuroda, H Laboratory of Microbiology, Kobe Women’s College of Pharmacy A polysaccharide was extracted from fruit bodies of Grifola frondosa ( maitake ), and the chemical structure and antitumor activity was studied. The extracted polysaccharide could be hydrolyzed by beta-glucanase into glucose, indicating it to be a beta-glucan. The sample gave methyl 2,3,4,6-tetra-O-methyl 2,4,6-tri-O-, methyl2,3,4-tri-O- and methyl 2,4-di-O-methylglucoside in...

[#1986-09] Maitake: Ohno, Naohito et al ...

Effect of Activation or Blockade of the Phagocytic System on the Antitumor Activity of Grifolan NAOHITO OHNO,a MAMI HAYASHI,a IWAO SUZUKI,a SHOZO OIKAWA,b KICHIRO SATO,b YOSHIYUKI Suzum,b and TOSHIRO YADOMAE” Tokyo College of Pharmacy,’ Horinouchi, Hachioji, Tokyo 192-03, Japan and Nippon Beet Sugar Mfg., Co., Ltd.,”Chuo-ku, Tokyo 104, Japan The effect of activation or blockade of the phagocytic system on the antitumor activity of grifolan (from maitake ) was investigated. The activity of grifolan was abrogated by treatment of mice with a blocker of the...

[#1986-08] Maitake and other mushrooms: ...

Carbon-13 Nuclear Magnetic Resonance Spectroscopy of Some Edible Mushrooms NAOHITO OHNO, IWAO SUZUKI, and TOSHIRO YADOMAE* Tokyo College of Pharmacy, Horinouchi, Hachioji, Tokyo 192-03, Japan In this paper, carbon-13 nuclear magnetic resonance (13C-NMR) spectra of some edible Mushrooms, Grifola frondosa ( maitake ), Lentinus edodes, Flammulina velutipes, Pholiota nameko, Agaricus bisporus, Lyophyllum cinerascens, Lyophyllum aggregatum, and Peziza vesiculosa, as aqueous suspensions were measured and the glucan compositions in these mushrooms were compared. The “C-NMR...

[#1986-07] Characterization of the Antit...

Characterization of the Antitumor Glucan Obtained from Liquid-Cultured Grifola frondosa Ohno, N (a), Adachi, Y (a), Suzuki, I (a), Sato, K (b), Oikawa, S (b), Yadomae, T(a) a) Tokyo College of Pharmacy, b) Nippon Beet Sugar Mfg. Co. Ltd. Chemical characterization of the extracts, and purification and structural characterization of an antitumor glucan obtained from liquid-cultured mycelium of Grifola frondosa ( maitake ) are described in this paper. The mycelium (13 g dry weight/l culture) was extracted successively with hot water (LMHW, 14.9 g/10g mycelium), cold alkali...

[#1986-06] Two Different Conformations o...

Two Different Conformations of Antitumor Glucans Obtained from Grifola frondosa Ohno, N (a), Adachi, A (a), Suzuki, A (a), Oikawa, S (b), Sato, K (b), Suzuki, Y (b), Ohsawa, M (b), Yadomae, T (a) a) Tokyo College of Pharmacy, b) Nippon Beet Sugar Mfg. Co. Ltd. Several antitumor glucan fractions obtained by various extraction procedures from Grifola frondosa ( maitake ) were compared by solution and solid (cross polarization, magic angle spinning (CP/MAS)) carbon-13 nuclear magnetic resonance (13C-NMR) spectroscopy. The fractions prepared under milder conditions, i.e. hot...