[#2010-21] Shiitake Itoh, Ayano et al. Hepatoprotective Effect of Syringic Acid and Vanillic Acid on CC14 Induced Liver Injury
Hepatoprotective Effect of Syringic Acid and Vanillic Acid on CC14 Induced Liver Injury
Ayano ITOH, a Katsuhiro ISODA, a Masuo KONDOH, a Malaya KAWASE, a Akihiro WATARI, a Masakazu KOBAYASHI,b Makoto TAMESADA,b and Kiyohito YAGI * ‘a
a Graduate School of Pharmaceutical Sciences, Osaka University; 1-6 Yamada-oka, Saha, Osaka 565-0871, Japan: and b Research and Development Center, Kobayashi Pharmaceutical Co., Ltd.; 1-30-3 Toyokawa, Ibaraki, Osaka 567-0057, Japan. Received December 22, 2009; accepted March 16, 2010; published online March 19, 2010
The mycelia of the edible mushroom Lentinula edodes ( shiitake ) can be cultured in solid medium containing lignin, and the hot-water extracts (L.E.M.) is commercially available as a nutritional supplement. During the cultivation, phenolic compounds, such as syringic acid and vanillic acid, were produced by lignin-degrading peroxidase secreted from L. edodes mycelia. Since these compounds have radical scavenging activity, we examined their protective effect on oxidative stress in mice with CCI4-induced liver injury. We examined the hepatoprotective effect of syringic acid and vanillic acid on CCI4-induced chronic liver injury in mice. The injection of CCI4 into the peritoneal cavity caused an increase in the serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. The intravenous administration of syringic acid and vanillic acid significantly decreased the levels of the transaminases. Four weeks of CCI4 treatment caused a sufficiently excessive deposition of collagen fibrils. An examination of Azan-stained liver sections revealed that syringic acid and vanillic acid obviously suppressed collagen accumulation and significantly decreased the hepatic hydroxyproline content, which is the quantitative marker of fibrosis. Both of these compounds inhibited the activation of cultured hepatic stellate cells, which play a central role in liver fibrogenesis, and maintained hepatocyte viability. These data suggest that the administration of syringic acid and vanillic acid could suppress hepatic fibrosis in chronic liver injury.
