[#2004-15] Shiitake, Royal Agaricus Edneia A. Souza-Paccola et al. ANTIMUTAGENIC ACTION OF LENTINULA EDODES AND AGARICUS BLAZEI ON ASPERGILLUS NIDULANS CONIDIA

ANTIMUTAGENIC ACTION OF LENTINULA EDODES AND AGARICUS BLAZEI ON ASPERGILLUS NIDULANS CONIDIA

Edneia A. Souza-Paccola‘; Cleide A. Bomfeti²; Léia C.L. Fávaro²; Inês C.B. Fonseca‘; Luzia D. Paccola-Meirelles²*

¹Departamento de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil

²Departamento de Ciências Biológicas, Universidade Estadual de Londrina,Londrina, PR, Brasil

ABSTRACT

The antimutagenic effect of the mushrooms Lentinula edodes ( Shiitake ) and Agaricus blazei was studied on conidia of Aspergillus nidulans when exposed to short wave ultraviolet light. Two strains of A. nidulans were used. For the preparation of the extracts, the fresh mushrooms were left in aqueous infusion for 12 hours and heated in a water bath for 15 min at 100ºC, and then the material was filtered. The dehydrated mushrooms were left in aqueous infusion for 12 hours and to filtrated. Both filtrates were used as extracts. A. nidulans conidia were incubated for three hours in water and in mushroom extracts and only after were exposed to UV light (pre­treatment). A. nidulans conidia were suspended in water and in mushroom extracts and immediately submitted to UV light (post-treatment). Conidial suspension in water and in mushroom extracts but without exposure to the mutagenic agent were used as controls. After mutagenic treatment, it was observed an increase in the survival rate of the A. nidulans and a decrease in the percentage of morphologic mutants on conidia treated with mushroom extracts. Our results demonstrated the radioprotective and antimutagenic effect of L. edodes and A. blazei mushrooms on eukaryotic cells when exposed to UV radiation.